Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA sequencing of human melanoma cells

ABSTRACT: RNA seq of siCtrl- and siSALL4-treated human melanoma cells (M010817)

PROVIDER: PRJEB39208 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR4318733.fastq.gz	Fastqsanger.gz
	ERR4318734.fastq.gz	Fastqsanger.gz
	ERR4318735.fastq.gz	Fastqsanger.gz
	ERR4318736.fastq.gz	Fastqsanger.gz
	ERR4318737.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

Next Generation Sequencing Facilitates Quantitative Analysis of Transcriptomes in Tbx2 depleted melanoma cells

Project description:Mouse melanoma B16 cells were transfected with either siCtrl of siTbx2 for 48 h, then RNA extracted and use in library preparation and following sequencing.

2022-01-07 | GSE175704 | GEO

ChIP sequencing for H3K27ac in melanoma cells

Project description:ChIP seq for H3K27ac in human melanoma cells (M010817) treated with siCtrl or siSALL4 siRNA for 48 hrs.

| PRJEB39209 | ENA

Genes regulated by DNMT1 in WM266.4 human melanoma cells

Project description:Gene expression data of WM266.4-Luc-mEGFP cells treated with siCtrl or siDNMT1

2020-05-15 | GSE150557 | GEO

RNA-sequencing of formalin fixed human primary melanoma tissue

Project description:RNA-sequencing of formalin fixed human primary melanoma tissue

| PRJNA520916 | ENA

RNA profiling of human lymphatic endothelial cells exposed to melanoma exosomes

Project description:In order to study the influence of melanoma-derived exosomes in the lymphatic vasculature within the lymph nodes, we have profile the transcriptional changes occurring in human lymphatic endothelial cells treated with melanoma-secreted exosomes or with the conditioned medium from melanoma cells depleted of exosomes.

2021-07-16 | GSE135187 | GEO

RNA Sequencing of si-Contol treated M010817 and siSMAD7 treated M010817 human melanoma cell line

Project description:RNA Sequencing of si-Contol treated M010817 and siSMAD7 treated M010817 human melanoma cell line

| PRJNA451514 | ENA

RNA-seq analysis of uveal melanoma cells and biopsy samples treated with FR900359

Project description:Uveal melanoma cell lines and short-term cultures of human uveal melanoma cells from biopsy samples were treated with 100 nM FR900359 or vehicle. RNA-seq was performed.

2021-02-15 | GSE165552 | GEO

RNA Sequencing of si-Contol treated M010817 and siSMAD7 treated M010817 human melanoma cell line

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived retinal transcriptome profiling (RNA-seq) to microarray and quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods and to evaluate protocols for optimal high-throughput data analysis Methods: mRNA profiles of si-Contol treated M010817 and siSMAD7 treated M010817 human melanoma cell line were generated by deep sequencing, in triplicate, using Illumina HiSeq 2500. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. qRT–PCR validation was performed using TaqMan and SYBR Green assays Conclusions: Our study represents the first detailed analysis of si-Contol treated M010817 and siSMAD7 treated M010817 human melanoma cell line transcriptomes, with biologic replicates, generated by RNA-seq technology. The optimized data analysis workflows reported here should provide a framework for comparative investigations of expression profiles. Our results show that NGS offers a comprehensive and more accurate quantitative and qualitative evaluation of mRNA content within a cell or tissue. We conclude that RNA-seq based transcriptome characterization would expedite genetic network analyses and permit the dissection of complex biologic functions.

2020-04-10 | GSE113472 | GEO