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ICLIP of Dazl in mouse embryonic stem cells

ABSTRACT: iCLIP of Dazl in mouse embryonic stem cells

PROVIDER: PRJEB39321 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR4331263.fastq.gz	Fastqsanger.gz

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iCLIP of Dazl in mouse embryonic stem cells

Project description:In this experiment we would like to identify the binding sites of Dazl on the transcriptome of mESCs. To do that, we carried out an iCLIP experiment using mESCs grown in 2iL condition.

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ChIP-Seq of Dazl in mouse embryonic stem cells

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ChIP-Seq of Dazl in mouse embryonic stem cells

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An RNA-interacting protein network regulates pluripotency in mammalian primordial germ cells (Dazl testis RNA-IP)

Project description:Primordial germ cells (PGCs) are the embryonic precursors to egg and sperm. When removed from the embryonic gonad, PGCs can give rise to embryonic germ cell lines (EGs), pluripotent stem cells that display most of the characteristics of embryonic stem cells (ESCs) including the ability to form teratomas and to contribute to chimera formation. In mice, EG cells can be derived between E8.5 up to E12.5 of embryonic development, at which point the PGCs undergo sexual differentiation and in the male transition into unipotent gonocytes. Dazl, a germ cell-specific RNA-binding protein, is specifically expressed in developing PGCs and is required for proper germ cell development. Dazl knockout mice are infertile, but the molecular mechanisms underlying this phenotype are still unknown. Here we demonstrate that Dazl localizes in granular structures in mammalian PGCs but not in ESCs. We demonstrate Dazl plays a central role in a large mRNA/protein interactive network that includes members of Fragile-X family RNA-binding proteins. We demonstrate that Dazl and Fxr1 play a central role in these granules and directly regulate the translation of specific core pluripotency factors, including Sox2 and Suz12. RNA species interacting specifically with Dazl in juvenile testicular germ cells were identified by RNA-IP microarray analysis. This dataset contains data from native RNA-IPs from P14 Dazl-GFP transgenic mouse testes. Native RNA-IP (anti-V5 and anti-PABP1) experiments from juvenile testicular germ cells expressing Dazl-GFP-V5. Input Total RNA and mock IP with normal mouse IgG were used as controls. Control and IP-enriched RNA samples were analyzed by Agilent Mouse Whole Genome 4X44K one-color microarrays. Two replicates for each condition were done.

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