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ABSTRACT: More than a methanotroph: a broader substrate spectrum for Methylacidiphilum fumariolicum SolV

PROVIDER: PRJEB39356 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR4760530.fastq.gz	Fastqsanger.gz
	ERR4762700.fastq.gz	Fastqsanger.gz
	ERR4766820.fastq.gz	Fastqsanger.gz
	ERR4760551.fastq.gz	Fastqsanger.gz
	ERR4760561.fastq.gz	Fastqsanger.gz

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Methylacidiphilum fumariolicum SolV

Project description:Methylacidiphilum fumariolicum SolV Raw sequence reads

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Methylacidiphilum fumariolicum SolV

Project description:Methylacidiphilum fumariolicum SolV Genome sequencing and assembly

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Metabolic regulation of ‘Ca. Methylacidiphilum fumariolicum’ SolV cells grown under different nitrogen and oxygen limitations

Project description:Aerobic methanotrophic bacteria can use methane as their sole energy source. The discovery of ‘Ca. Methylacidiphilum fumariolicum’ strain SolV and other verrucomicrobial methanotrophs has revealed that the ability of bacteria to oxidize CH4 is much more diverse than has previously been assumed in terms of ecology, phylogeny and physiology. A remarkable characteristic of the methane-oxidizing Verrucomicrobia is their extremely acidophilic phenotype, growing even below pH 1. In this study we used RNA-Seq to analyze the metabolic regulation of ‘Ca. M. fumariolicum’ SolV cells growing at μmax in batch culture or under nitrogen fixing or oxygen limited conditions in chemostats, all at pH 2. The analysis showed that two of the three pmoCAB operons each encoding particulate methane monoxygenases were differentially expressed, probably regulated by the available oxygen. The hydrogen produced during N2 fixation is apparently recycled as demonstrated by the upregulation of the genes encoding a Ni/Fe-dependent hydrogenase. These hydrogenase genes were also upregulated under low oxygen conditions. Handling of nitrosative stress was shown by the expression of the nitric oxide reductase encoding genes norB and norC under all conditions tested, the upregulation of nitrite reductase nirK under oxygen limitation and of hydroxylamine oxidoreductase hao in the presence of ammonium. Unraveling the gene regulation of carbon and nitrogen metabolism helps to understand the underlying physiological adaptations of strain SolV in view of the harsh conditions of its natural ecosystem.

2012-09-01 | GSE40528 | GEO

Metabolic regulation of ‘Ca. Methylacidiphilum fumariolicum’ SolV cells grown under different nitrogen and oxygen limitations

2012-09-01 | E-GEOD-40528 | biostudies-arrayexpress

Genome Methylacidiphilum fumariolicum novel annotation

Project description:The genome of Methylacidiphilum fumariolicum SolV was analysed in detail and annotated

| PRJEB59974 | ENA

Candidatus Methylacidiphilum fumarolicum

Project description:Methylacidiphilum fumariolicum overview

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Project description:methylacidiphilum fumariolicum transcriptome reads

| PRJNA766544 | ENA

Candidatus Methylacidiphilum fumarolicum

Project description:Metabolic regulation of ‘Ca. Methylacidiphilum fumariolicum’ SolV cells grown under different nitrogen and oxygen limitations

| PRJNA174222 | ENA

Candidatus Methylacidiphilum fumarolicum strain:Pic

Project description:Methylacidiphilum fumariolicum strain Pic Genome sequencing and assembly

| PRJNA749370 | ENA

Heterotrophic growth on formate is dependent on the maintenance of intracellular pH for the thermoacidophilic methanotroph Methylacidiphilum sp RTK17.1.

Project description:In this work, we investigated intracellular pH homeostasis within the thermoacidophilc methanotroph Methylacidiphilum sp. RTK17.1. Our findings show the proton motive force for this species is primarily generated by a pH gradient across the cellular membrane. In batch experiments, the addition of formate resulted in no observable cell growth and, correspondingly, acidification of the cytosol, decreased formate dehydrogenase activity and (presumably) cell-death. Nevertheless, we were able to demonstrable growth on formate as the sole source of metabolizable energy was possible in steady-state (continuous) cultures following the transition from methanol to formate. Under these conditions, biomass productivity yields on formate were 63% less than for growth on methanol. Transcriptome analysis revealed key genes associated with pH homeostasis, methane, methanol and formate metabolism were significantly regulated in response to growth on formate. Collectively, these results suggest environmental formate represents a utilisable source of energy/carbon to the acidophilic methanotrophs during periods of methane starvations and highlights potential short-comings of traditional batch-culture physiological characterisation studies in acidophilic species.

2020-10-31 | GSE145277 | GEO

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