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3'-UTR-seq of female and male mouse pituitary gland across pubertal transition

ABSTRACT: 3'-UTR-seq of female and male mouse pituitary gland across pubertal transition

PROVIDER: PRJEB39964 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR4471790.fastq.gz	Fastqsanger.gz
	ERR4471800.fastq.gz	Fastqsanger.gz
	ERR4471810.fastq.gz	Fastqsanger.gz
	ERR4471820.fastq.gz	Fastqsanger.gz
	ERR4471830.fastq.gz	Fastqsanger.gz

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3'-UTR-seq of female and male mouse pituitary gland across pubertal transition

Project description:The pituitary gland exhibits sex differences in its function. Diseases associated with dysregulation of the pituitary are also sex-biased in prevalence. Previous qPCR profiling of puberty-related genes in the pituitary gland revealed increasingly sex-biased expression of genes profiled across pubertal transition. Here, we performed 3’-UTR-seq on pituitary gland from both sexes of C57BL/6J mice at 5 ages spanning pubertal transition (postnatal days 12, 22, 27, 32, 37) (6 replicates per sex at each age) to examine genome-wide sex-biased trends in gene expression and potential regulatory mechanisms of these sex differences. QuantSeq 3’mRNA-seq libraries were constructed from total RNA using an automated method with Agilent NGS Workstation. Resulting single-end libraries were sequenced at SickKids TCAG core on the Illumina v4 flow cell with SR50 bp cycles extended to 68 bp. A customized pipeline was developed and used for analysis of reads obtained (see paper for details). Processed reads were mapped to mouse genome (mm10).

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