Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Brn2 (POU3F2) ChIP-seq data obtained from asynchronous or mitotically arrested mouse neural stem/progenitor cells

ABSTRACT: Brn2 (POU3F2) ChIP-seq data obtained from asynchronous or mitotically arrested mouse neural stem/progenitor cells

PROVIDER: PRJEB41287 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR4832150.fastq.gz	Fastqsanger.gz
	ERR4832160.fastq.gz	Fastqsanger.gz
	ERR4832170.fastq.gz	Fastqsanger.gz
	ERR4832151.fastq.gz	Fastqsanger.gz
	ERR4832161.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 32

Similar Datasets

Brn2 (POU3F2) ChIP-seq data obtained from asynchronous or mitotically arrested mouse neural stem/progenitor cells

Project description:dataset contains 8 samples: 2 asynchronous and 2 mitotically synchronized populations (one with 90% and another with 95% mitotic purity) and 4 corresponding inputs. Cells were synchonized for 10h with colchicine at 330nM. asyncronous samples (interphase) were sonicated and prepared in parallel with same cell number as the corresponding mitotic samples. Fetal neural stem/progenitor cells were obtained from mouse telencephalon (ventral region) at E13.5. This data generated 4 genome-wide binding profiles corresponding to Brn2 (POU3f2) binding sites, aligned to MM9 mus musculus dataset.

2021-02-13 | E-MTAB-9758 | biostudies-arrayexpress

Analysis of Pol II position in asynchronous, G1- or G2/M-arrested cells

Project description:We sequenced RNA Pol II Flag pulldowns (NET-seq) from five samples of W303 bar1D (asynchronous, alpha-Factor arrested, nocodazole arrested or transformed with a pGAL1 multicopy plasmid carrying either no ORF or the SEN1 ORF)

2018-04-12 | GSE86419 | GEO

Genomic RNA-DNA duplexes in mitotically arrested cells

Project description:Genomic RNA-DNA duplexes in mitotically arrested cells

| PRJNA685178 | ENA

Budding yeast cohesin (Mcd1) distribution in mitotically arrested cells

Project description:genomic localization of the budding yeast cohesin complex was mapped in mitotically arrested cells by Mcd1 ChIP followed by hybridization to high-density tiled microarrays

2009-10-01 | GSE18021 | GEO

Budding yeast cohesin (Mcd1) distribution in mitotically arrested cells

Project description:genomic localization of the budding yeast cohesin complex was mapped in mitotically arrested cells by Mcd1 ChIP followed by hybridization to high-density tiled microarrays Cells were synchronized first in G1 and then released into media containing the microtubule poison nocodazole. Cells were fixed and processed for ChIP once arrested as large-budded cells. Immunoprecipitated DNA and total genomic DNA not subjected to immunoprecipitation were competitively hybridized to Nimblegen whole genome arrays.

2009-09-30 | E-GEOD-18021 | biostudies-arrayexpress

Optogenetic induction of Brn2 drive neural differentiation

Project description:We use optical induction of Brn2 to probe mechanisms for gating embryonic stem cell differentiation mRNA-seq time-course following Brn2 induciton

2015-08-26 | E-GEOD-71889 | biostudies-arrayexpress

BRN2 expression increases anoikis resistance in melanoma.

Project description:Melanoma tumors are highly heterogeneous, comprising of many cell populations that vary in their potential for growth and invasion. Differential transcription factor expression contributes to these phenotypic traits. BRN2, a member of the POU domain family of transcription factors is thought to play important roles in melanoma invasion and metastasis. However, how BRN2 functions during the metastatic process of melanoma remains largely unknown. We therefore investigated the effects of BRN2 expression in melanoma cells with no or low constitutive expression using a doxycycline-inducible system. Induction of BRN2 expression led to reduced proliferation and partial resistance to an inhibitor of mutated BRAF. Whole genome profiling analysis revealed novel targets and signaling pathway changes related to prevention of cell death induced by detachment from the extracellular matrix, known as anoikis resistance. Further investigation confirmed increased survival of BRN2 expressing cell lines in non-adherent conditions. Functionally, expression of BRN2 promoted induction of c-MET levels as well as increased phosphorylation of STAT3. Treatment with crizotinib, a c-MET inhibitor, decreased cellular viability of BRN2 expressing cells under non-adherent conditions to death by anoikis. These results highlight the importance of a largely overlooked transcription factor in the progression and metastasis of melanoma, and may suggest a strategy to target BRN2 expressing cells resistant to therapy and cell death by anoikis.

2020-02-25 | GSE145806 | GEO

CRAC of yeast RNA polymerase II and RNases H in asynchronous, G1-arrested, and S-phase cells.

Project description:CRAC of yeast RNA polymerase II and RNases H in asynchronous, G1-arrested, and S-phase cells.

| PRJNA802688 | ENA

ChIP-Seq of Sox2 and Brn2 in ESCs, NPCs, and differentiating ESCs

Project description:We analyzed the genome-wide binding of Sox2 and POU factor partner factors, Oct4 in ESCs (using published datasets PMID:18692474 and GSM307137, GSM307154, GSM307155) and Brn2 in NPCs. We found that Sox2 and Oct4 co-occupied a large subset of promoters and enhancers in ESCs, but that Sox2 and Brn2 co-occupy predominantly enhancers. Further, we overexpressed Brn2 in differentiating ESCs and showed that ectopic Brn2 recruited Sox2 to NPC-specific targets, resulting in skewed differentiation towards the neural lineage. Examination of transcription factor binding in ESCs, NPCs, and differentiating ESCs by ChIP-Seq.

2013-02-20 | E-GEOD-35496 | biostudies-arrayexpress

ChIP-Seq of Sox2 and Brn2 in ESCs, NPCs, and differentiating ESCs

2013-02-20 | GSE35496 | GEO