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IlMamBras1

ABSTRACT: Mamestra brassicae (cabbage moth) genome assembly, ilMamBras1

PROVIDER: PRJEB42032 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	CAJHZQ01.dat.gz	Other
	CAJHZQ01.fasta.gz	Fasta.gz
	CAJHZQ01.master.dat	Other

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ilMamBras

Project description:Mamestra brassicae (cabbage moth)

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ilMamBras1 alternate haplotype

Project description:Mamestra brassicae (cabbage moth) genome assembly, ilMamBras1, alternate haplotype

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ilMamBras

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Gene expression in Arabidopsis thaliana leaves after chilling and/or herbivory by Pieris brassicae or Mamestra brassice larvae or after mechanical wounding

Project description:To investigate the impact of a plant´s response to abiotic stress on plant defense against subsequent biotic stress, we determined the transcriptional response of Arabidopsis thaliana to low temperature stress (4°C) and subsequent mechanical wounding or larval feeding damage by the herbivores Mamestra brassicae (generalist) and Pieris brassicae (specialist). In total, 21%, 4% and 14% of all genes responsive to M. brassicae, P. brassicae or mechanical wounding were differentially regulated in previously cold-treated compared to untreated plants.

2019-05-15 | GSE114211 | GEO

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Brassica napus

Project description:Activation of plant defence in Brassica napus L. cv. Westar and transgenic MINELESS plants after attack by Mamestra brassicae (cabbage moth)

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Activation of plant defence in Brassica napus L. cv. Westar and transgenic MINELESS plants after attack by Mamestra brassicae (cabbage moth)

Project description:Plants within the Brassicaceae family have a unique defence mechanism known as the “glucosinolate-myrosinase” system. Upon tissue disruption by insect herbivores, glucosinolates are hydrolysed by the enzyme myrosinase (EC 3.2.1.147) into a variety of degradation products, which can deter insect herbivory. This process has been termed as “The Mustard Oil Bomb”. Seeds of Brassica napus have been genetically modified to remove myrosinase containing myrosin cells. The modified plants have been named MINELESS due to a lack of toxic mines in seeds. This study aimed to get insights into defence responses of B. napus wild-type and MINELESS seedlings, after being challenged by larvae of the generalist herbivore Mamestra brassicae. The microarray analysis showed 494 and 159 genes to be differentially regulated after M. brassicae feeding on wild-type and MINELESS seedlings, respectively. Many of the observed transcriptional responses i B. napus and the MINELESS mutant are related to those found in Arabidopsis thaliana plants when they are exposed to insects.

2015-01-01 | GSE40932 | GEO

Genome assembly and annotation of Brevicoryne brassicae

Project description:A chromosome-level genome assembly of the cabbage aphid Brevicoryne brassicae

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Revised annotation of Plutella xylostella microRNAs

Project description:We used our newly ultra deep sequence data and bioinformatics to re-annotate P. xylostella genome for high confidence miRNAs with the correct 5p and 3p arm features. Furthermore, the whole genome was screened to identify potential miRNA binding sites using three target-predicting algorithms. Totally, 203 mature miRNAs were annotated, including 33 novel miRNAs. Two geographical populations of Diamondback moth larvae from Queensland (Gatton) and South Australia (Waite) were collected and reared on the cabbage plant at the University of Queensland in Australia. Total RNA was extracted from fifteen 3rd instar larval samples using TriazolÂ® following the manufacturerâs protocol (Life Technologies). The small RNA libraries were generated from both populations with three biological replicates using the Illumina Truseq small RNA preparation kit at the Australian Genome Research Facility (AGRF-Melbourne, Australia). The purified cDNA libraries were sequenced on Illumina HiSeq and raw sequencing reads (50 nt) were obtained using Illuminaâs Sequencing Control Studio software.

2016-08-01 | E-GEOD-76321 | biostudies-arrayexpress

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