Project description:High-throughput sequencing of Arabidopsis thaliana endogenous small RNAs by 454 pyrosequencing. Keywords: high-throughput sequencing

2006-11-02 | GSE5228 | GEO

High-throughput sequencing of small RNAs from Drosophila

Project description:High-throughput sequencing of Drosophila melanogaster small RNAs. total RNA, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH Keywords: high-throughput 454 sequencing

2007-06-10 | GSE7448 | GEO

Clinical trial of human Solid Tumor Multi-gene Mutation Combined Detection Kit (High-throughput sequencing)

Project description:Interventions: Gold Standard:Reference methods: 1. Total exome sequencing (WES) 2. Multiple fluorescence PCR capillary electrophoresis (PCR-STR); Comparison method: comparison of similar kits;Index test:Information of reagents to be examined: (1) Product name: Human solid Tumor Multi-gene Mutation Combined Detection Kit (high-throughput sequencing method); (2) Packing specification: 48 test/box; (3) Storage conditions: Kit 1 was stored at -25~-15?, Kit 2 at -85~-70?, Kit 3 at 2-8 ?, kit 4 at 10-30 ?; (4)&# Primary outcome(s): Non-small cell lung cancer/colorectal cancer/gastrointestinal stromal tumor/stomach cancer/glioma/thyroid cancer/breast cancer/ovarian cancer/melanoma/cholangiocarcinoma/urothelial carcinoma/prostate cancer/other malignant solid tumor genes Study Design: Sequential

| 36510 | ecrin-mdr-crc

FragSeq: transcriptome-wide RNA structure probing using high-throughput sequencing.

Project description:To accelerate previous RNA structure probing approaches, which focus on analyzing one RNA sequence at a time, we have developed FragSeq, a high-throughput RNA structure probing method that uses high-throughput RNA sequencing to identify single-stranded RNA (ssRNA) regions from fragments generated by nuclease P1, which is specific for single-stranded nucleic acids. In the accompanying study, we show that we can accurately and simultaneously map ssRNA regions in multiple non-coding RNAs with known structure in experiments probing the entire mouse nuclear transcriptome. We carried out probing in two cell types to assess reproducibility. We also identified and experimentally validated structured regions in ncRNAs never previously probed.

2010-11-07 | GSE24622 | GEO

Expression profiling by high throughput sequencing

Project description:Expression profiling by high throughput sequencing

| PRJEB7300 | ENA

High-throughput sequencing of strawberry fruits

Project description:High-throughput sequencing of strawberry fruits

| PRJNA860530 | ENA

X chromosome by high throughput sequencing

Project description:X chromosome by high throughput sequencing

| PRJNA661456 | ENA