Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure

ABSTRACT: Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure

PROVIDER: PRJEB43297 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other
	ERR5353540.fastq.gz	Fastqsanger.gz
	ERR5353550.fastq.gz	Fastqsanger.gz
	ERR5353560.fastq.gz	Fastqsanger.gz
	ERR5353570.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 713

Similar Datasets

Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part)

Project description:Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part)

| PRJEB43299 | ENA

Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure (small RNA part)

Project description:Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure (small RNA part)

| PRJEB43296 | ENA

Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure

Project description:We then utilized parallel single-cell RNA sequencing and bisulfite sequencing to explore the impact of GC exposure on the human embryo. In addition, to further explore the role of epigenetic regulation as a mechanism for the observed transcriptional changes, we examined the effects of GCs on small non-coding RNA using smallseq.

2022-08-02 | E-MTAB-10096 | biostudies-arrayexpress

Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part)

2022-08-02 | E-MTAB-10097 | biostudies-arrayexpress

Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure (small RNA part)

2022-08-02 | E-MTAB-10098 | biostudies-arrayexpress

Whole blood DNA methylation profiling of glucocorticoid excess

Project description:Evaluation of whole blood DNA methylome in 94 samples obtained from 47 patients with different glucocorticoid states, including Cushing’s syndrome, eucortisolism and adrenal insufficiency, in order to identify a molecular signature reflecting glucocorticoid action.

2022-01-19 | E-MTAB-10092 | biostudies-arrayexpress

Effects of Hsd11b1 overexpressing in mc3T3-e1 pre-osteoblastic cell lines under glucocorticoid excess

Project description:Effects of Hsd11b1 overexpressing in mc3T3-e1 pre-osteoblastic cell lines under glucocorticoid excess

| PRJNA1148064 | ENA

Phosphate excess increases susceptibility to pathogen infection in rice

Project description:Phosphorus (P) is an essential nutrient for plant growth and productivity. Due to soil fixation, however, phosphorus availability in soil is rarely sufficient to sustain high crop yields. Fertilizers are widely used to circumvent the limited bioavailability of phosphate (Pi) which led to a scenario of excessive soil P in agricultural soils. Whereas adaptive responses to Pi deficiency have been deeply studied, less is known about how plants adapt to Pi excess and how Pi excess might affect disease resistance. Here, we show that high Pi fertilization in rice plants, and subsequent Pi accumulation in leaves, enhances susceptibility to infection by Magnaporthe oryzae, the causal agent of the rice blast disease. Equally, MIR399f overexpression causes an increase in Pi content in rice leaves which results in enhanced susceptibility to M. oryzae. During pathogen infection, a weaker activation of defense-related genes occurs in rice plants accumulating Pi in leaves, a response that is in agreement with the phenotype of blast susceptibility observed in these plants. These data support that Pi, when in excess, compromises defense mechanisms in rice while demonstrating that miR399 functions as a negative regulator of rice immunity. The two signaling pathways, Pi signaling and defense signaling, must operate in a coordinated manner in controlling disease resistance. This information provides a basis to understand the molecular mechanisms involved in immunity in rice plants grown under a high Pi fertilization regime, an aspect that should be considered in management of the rice blast disease

2020-03-16 | GSE137735 | GEO

Single-cell multi-omics of human preimplantation embryos shows susceptibility to glucocorticoids.

Project description:The preconceptual, intrauterine, and early life environments can have a profound and long-lasting impact on the developmental trajectories and health outcomes of the offspring. Given the relatively low success rates of assisted reproductive technologies (ART; ∼25%), additives and adjuvants, such as glucocorticoids, are used to improve the success rate. Considering the dynamic developmental events that occur during this window, these exposures may alter blastocyst formation at a molecular level, and as such, affect not only the viability of the embryo and the ability of the blastocyst to implant, but also the developmental trajectory of the first three cell lineages, ultimately influencing the physiology of the embryo. In this study, we present a comprehensive single-cell transcriptome, methylome, and small RNA atlas in the day 7 human embryo. We show that, despite no change in morphology and developmental features, preimplantation glucocorticoid exposure reprograms the molecular profile of the TE lineage, and these changes are associated with an altered metabolic and inflammatory response. Our data also suggest that glucocorticoids can precociously mature the TE sublineages, supported by the presence of extravillous trophoblast markers in the polar sublineage and presence of X Chromosome dosage compensation. Further, we have elucidated that epigenetic regulation-DNA methylation and microRNAs (miRNAs)-likely underlies the transcriptional changes observed. This study suggests that exposures to exogenous compounds during preimplantation may unintentionally reprogram the human embryo, possibly leading to suboptimal development and longer-term health outcomes.

| S-EPMC9528977 | biostudies-literature

Phosphate excess increases susceptibility to pathogen infection in rice

Project description:Phosphate excess increases susceptibility to pathogen infection in rice

| PRJNA566419 | ENA