Isolation and identification of Alternaria species from diseased potato in France
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PROVIDER: PRJEB43452 | ENA |
REPOSITORIES: ENA
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Project description:Isolation of different Alternaria speices from diseased tomato in Pakistan
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Project description:Study of Alternaria solani inoculation of wild type, Nahg transgenes and coiRNA potato
2020-10-01 | E-MTAB-8477 | biostudies-arrayexpress
Project description:Potato seedlings were subjected to cold, heat and salt stress. Expression profiles were captured at three different time-points, 3h, 9h and 27h from two different tissues, roots and leaves. The experiment was preformed independently three times. Commercially available true potato seeds (Variety Gilroy) were germinated on rafts floating on hydroponic medium in Magenta boxes. Plants were grown for 5 weeks prior to stress application under long day conditions (16h light and 8h dark) at 25C with gentle agitation. To initiate stress the medium was replaced with fresh medium pre-chilled to 4C (cold stress), pre-heated to 35C (heat stress) or supplemented with 100mM NaCl (salt stress). Cold and heat stress were maintained for the duration of the experiment by placing the Magenta boxes on ice or in a water-bath at 35C. For every individual sample two boxes of plants were used pooling a total of 6 plants per sample. For each time-point a single control sample was used by changing the media in a similar way as for the stress induction. A total of six boxes were combined for the pooled reference samples. Plants were harvested at the appropriate time and snap-frozen in liquid nitrogen. Roots and aerial tissue was separated prior to freezing. The tissue was stored at -80C freezer until isolation. Total RNA was isolated using RNeasy isolation kit. The intactness of the RNA was verified on gel and the concentration was adjusted to 3ug/ul by ethanol precipitation and re-suspension. Series_weblink: http://www.tigr.org/tdb/potato Keywords = potato, Abiotic stress Keywords: ordered
2005-03-19 | GSE2416 | GEO
Project description:In the present study molecular interactions between potato plants, Colorado potato beetle (CPB) larvae and Potato virus YNTN (PVYNTN) were investigated by analyzing gene expression in potato leaves. Grant ID: J4-4165 Slovenian Research Agency ARRS Growth and defense trade-offs in multitrophic interaction between potato and its two major pests Grant ID: P4-0165 Slovenian Research Agency ARRS Biotechnology and Plant Systems Biology
2014-10-23 | GSE56333 | GEO
Project description:Alternaria, a widespread fungal genus, infects a wide range of crops and produces various toxins, such as Tentoxin (TEN), Tenuazonic acid (TeA), Alternariol (AOH) during infection, leading to a major concern about safety of these crops. Although the toxin-producing ability varies among different Alternaria species, the underlying mechanisms are still unclear. In this study, several Alternaria toxins were frequently detected in Tibetan barley (Hordeum vulgare var. coeleste Linnaeus) and wheat (Triticum aestivum Linn) in Tibet, especially TEN and TeA, which were detected in half of the crops. 153 barley and wheat samples collected from 5 different regions in Tibet were used for toxin detection/quantification, isolation and identification of the corresponding toxin producing fungi. According to levels of toxins produced in vivo, different isolates of Alternaria alternaria recovered from the above collected sampled were divided into three groups designated as high toxin-producing strains (HT), medium toxin-producing strains (LT) and no toxin-producing strains (NT). Metabolomics and transcriptomics were employed to investigate the potential mechanism of variations in toxin production among different isolates. Metabolomics analysis revealed high levels of amino acids and secondary metabolites in HT. Various precursors of TeA/TEN were also highly accumulated in HT, especially isoleucine ( which was one of essential gradients of TEN biosynthesis, which may account for high toxin production in HT strains in metabolome. Further transcriptome analysis revealed that the expression levels of genes relevant to precursors of TEN/TeA were higher in HT, especially CC77DRAFT_360136, CC77DRAFT_546209, CC77DRAFT_1033655 and CC77DRAFT_1090532, showing the molecular basis for toxin-producing variations among three strains. Importantly, our topological regulation network results identified the candidate genes that regulated these four genes involved in toxin-producing, and these genes may be essential for variations in toxin producing ability of these Alternaria strains. Overall, this comprehensive analysis expects to provide a better understanding to decipher the mechanism of toxicity based on molecular and metabolic levels.
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Project description:Transcriptomics Sequencing Provides Insights into Understanding the Infection Mechanism of Alternaria solani on Potato
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Project description:Comparative genomics of Alternaria alternata and Alternaria gaisen causing Alternaria brown spot of citrus in China
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Project description:The mkkkC5 mutant, a knockout line of MAPKKKC5, shows changes in growth and development, as well as an altered Alternaria sensitivity. It was shown by Brader and Djamei et al.(2007) that Alternaria brassicicola sensitivity is also influenced by MKK2-activity. Aim of this experiment is to access the transcriptional changes in the mkkkc5plants as well as in plants overexpressing a constitutively active MKK2-version (MKK2EE). The second part of the experiment addresses transcriptional changes in these plants after Alternaria brassicicola infection. au07-09_alternaria Keywords: treatment variations
2009-02-15 | GSE14443 | GEO
Project description:Plants have a wide variety of ways to defend against pathogens. To assist efforts to counter severe losses of Solanum tuberosum (potato) yields caused by various pathogens, we have studied immune responses of potato plants using quantitative proteomic techniques. We have combined protein isolation and subcellular fractionation to identify proteins that change in abundance during the immune response. This dataset contains the analysis of subcellular fraction 2 of our experiment. In a previous analysis, another fraction was analyzed using a different methodology. For this reason, the two datasets are submitted separately.
2024-05-31 | PXD038421 | Pride