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Chromatin accessibility in mouse hypothalamic arcuate nucleus in puberty onset

ABSTRACT: Chromatin accessibility in mouse hypothalamic arcuate nucleus in puberty onset

PROVIDER: PRJEB43863 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR5529245_1.fastq.gz	Fastqsanger.gz
	ERR5529245_2.fastq.gz	Fastqsanger.gz
	ERR5529246_1.fastq.gz	Fastqsanger.gz
	ERR5529246_2.fastq.gz	Fastqsanger.gz

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Chromatin accessibility in mouse hypothalamic arcuate nucleus in puberty onset

Project description:To better understand the epigenetic mechanism underlying pubertal onset, the hypothalamic genome-wide chromatin accessibility patterns in mouse arcuate nucleus at early and late pubertal stages were explored. Female mice have been widely used in multiple studies on pubertal development as they present the similar molecular behaviors in HPG axis and stable cycles of menstrual calendar like human. Hypothalamic ARC underwent a huge epigenetic and genetic reprogramming to adapt to the response and feedback on sexual hormones during the stages of early pubertal (2-5-week of age) and late puberty (5-8-week of age) . We harvested 4- and 8-week hypothalamic ARC and employed ATAC-seq on a genome-wide scale. Combined with previous RRBS, RRHP and RNA-seq, the connections between DNA (hydroxyl)methylation in retroelements and gene expression were studied, emphasizing the importance of epigenetic alterations in regulating transcription in puberty onset.

2021-04-02 | E-MTAB-10263 | biostudies-arrayexpress

Transcriptome and DNA (hydro)methylation in mouse hypothalamic arcuate nucleus in puberty onset

Project description:To better understand the epigenetic mechanism underlying pubertal onset, the hypothalamic genome-wide DNA methylation and hydroxymethylation patterns as well as the transcription profiles in mouse arcuate nucleus at early and late pubertal stages were explored. Female mice have been widely used in multiple studies on pubertal development as they present the similar molecular behaviors in HPG axis and stable cycles of menstrual calendar like human. Hypothalamic ARC underwent a huge epigenetic and genetic reprogramming to adapt to the response and feedback on sexual hormones during the stages of early pubertal (2-5-week of age) and late puberty (5-8-week of age) . We harvested 4- and 8-week hypothalamic ARC and employed RNA-seq, reduced representation bisulfite sequencing (RRBS) and hydroxymethylation profiling (RRHP) on a genome-wide scale. We identified a large number of differential expressed genes (DEGs) and differential 5(h)mC signals across the whole genome. We discovered novel connections between DNA (hydroxyl)methylated modification and gene expression, emphasizing the importance of epigenetic alterations in regulating transcription in puberty onset.

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Transcriptome and DNA (hydro)methylation in mouse hypothalamic arcuate nucleus in puberty onset.

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Transcriptome and DNA (hydro)methylation in mouse hypothalamic arcuate nucleus in puberty onset.

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Transcriptome and DNA (hydro)methylation in mouse hypothalamic arcuate nucleus in puberty onset

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Transcriptome-scale spatial gene expression in rat arcuate nucleus during puberty

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