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RNA-seq of human colonic cancer cell line CACO-2 transfected 48h with siRNA targeting JAK1 (HJ1D8 HJ1D2) or JAK3 (HJ3D41 HMJ3D1) at 100pM final concentration using LipofectamineRNAiMAX or treated 48h with Tofacitinib (final concentration: 1µM) or Filgotinib (final concentration: 5µM) against controls (transfection medium: Opti-MEM, transfection mixt alone: Opti-MEM + Lipof., siMock, DMSO equivalent to volume added for Filgo 5µM).

ABSTRACT: RNA-seq of human colonic cancer cell line CACO-2 transfected 48h with siRNA targeting JAK1 (HJ1D8 HJ1D2) or JAK3 (HJ3D41 HMJ3D1) at 100pM final concentration using LipofectamineRNAiMAX or treated 48h with Tofacitinib (final concentration: 1µM) or Filgotinib (final concentration: 5µM) against controls (transfection medium: Opti-MEM, transfection mixt alone: Opti-MEM + Lipof., siMock, DMSO equivalent to volume added for Filgo 5µM).

PROVIDER: PRJEB44249 | ENA |

REPOSITORIES: ENA

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RNA-seq of human colonic cancer cell line CACO-2 transfected 48h with siRNA targeting JAK1 (HJ1D8; HJ1D2) or JAK3 (HJ3D41; HMJ3D1) at 100pM final concentration using LipofectamineRNAiMAX or treated 48h with Tofacitinib (final concentration: 1µM) or Filgotinib (final concentration: 5µM) against controls (transfection medium: Opti-MEM, transfection mixt alone: Opti-MEM + Lipof., siMock, DMSO equivalent to volume added for Filgo 5µM).

Project description:Inflammatory bowel diseases are highly debilitating conditions that require constant monitoring and life-long medication. Current treatments are focused on systemic administration of immunomodulatory drugs, but they have a broad range of undesirable side-effects. The RNA interference is a highly specific endogenous mechanism that regulates the expression of the gene at the transcript level, which can be repurposed using exogenous short interfering RNA (siRNA) in order to repress the target gene’s expression. While siRNA therapeutics can offer an alternative to existing therapies, with a high specificity critical for chronically administrated drugs, evidence of their potency compared to chemical kinase inhibitors used in clinics is still lacking in alleviating an adverse inflammatory response. In this experiment, we compared the transcriptomic profile of cells transfected 48hv by siRNA targeting JAK1 and JAK3 (100 pM) to those of cells treated 48h with the most recent and promising kinase inhibitors for Janus kinases (Jakinibs), Tofacitinib (1 µM) and Filgotinib (5 µM).

2023-04-12 | E-MTAB-10348 | biostudies-arrayexpress

EWS-FLI1-transfection of HEK293

Project description:Response of HEK293-cells after transfection with EWS-FLI1. HEK293 cells were transfected with the expression vector pIRES2-EGFP containing type I EWS-FLI1 or empty control vector. For transient transfection standard DEAE dextran method was used and RNA was isolated 48h post transfection. For stable transfection cells were transfected using FuGENE 6 (Roche, Mannheim, Germany) and cells were selected with 400 ug/mL G418. DNA-microarray analysis was performed using Affymetrix HG-U133A microarrays.(see Staege et al. Cancer Res. 2004) Keywords = HEK293 Keywords = EWS-FLI1 Keywords = Ewing family tumors Keywords: other

2004-10-07 | GSE1822 | GEO

MLE12 cell transcriptome analysis 12, 24, 48h after Notch 1-ICD overexpression with NICD1-pIRES-dsRed2 vector

Project description:To study the effect of Notch 1 overexpression on cell funtion and to elucidate involved signalling pathways MLE12 cells tranfected with NICD1-pIRES-dsRed2 vector were analyzed for their transcript profiles 12, 24, and 48h after transfection and related to an empty vector (EV) transfected control cells

2018-03-01 | GSE95856 | GEO

Effects of plasmids transfection on the RO82_W1 human thyroid cell line

Project description:The goal of this study was to identifiy cellular pathways modified by RO82_W1 cell transfection with PRC, ERRalpha or PRC+ERRalpha plamids relative to transfection with PGl3 plasmid In duplicate, cells transfected with PRC, ERRalpha or PRC+ERRalpha plamids during 48h compared to PGl3 treated cells

2012-05-31 | E-GEOD-37240 | biostudies-arrayexpress

Final discharge from palm oil mill

Project description:Bacterial community in different POME final discharge

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Final discharge from palm oil mill

Project description:Bacterial community in different POME final discharge

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48h Immobilization in human

Project description:Our results suggest that 48h of immobilization increases expression of mRNA for components of the UPP and MT function while decreasing mRNA and protein for components involved in ECM integrity. We hypothesized that 48h of immobilization would increase gene expression and respective protein products for components of the ubiquitin proteasome pathway (UPP). Keywords: stimulus or stress design

2006-06-20 | GSE5110 | GEO

Gene expression in astrocytes upon sustained GSK-3 inhibition by small molecule

Project description:In order to identify molecular targets that mediates the stimulation of astrocyte's migration upon sustained specific inhibition of GSK-3, we have employed whole genome microarray expression profiling. Murine cortical-striatal astrocytes grown in primary culture were treated in vitro for 48 h with either 1 micromolar Ro3303544, or control medium (final equivalent concentration of DMSO), or washed-out for 48h in control medium after an initial 48h treatment with 1 micromolar Ro3303544.

2011-09-01 | GSE25770 | GEO

RNAseq of HCT116 p53KO cells transfected with either siRNA silencing ZNF84 or with negative control siRNA, in 2 variants of experiment a) with doxorubicin treatment (doxorubicin added 48h post-transfection, for the next 48h before harvesting) b) untreated, cultured 72h post-transfection

Project description:RNAseq of HCT116 p53KO cells transfected with either siRNA silencing ZNF84 or with negative control siRNA, in 2 variants of experiment a) with doxorubicin treatment (doxorubicin added 48h post-transfection, for the next 48h before harvesting) b) untreated, cultured 72h post-transfection

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Gene expression microarray analysis following siRNA knockdown of T-UC.300A

Project description:SH-SY5Y cells were transfected with an siRNA against T-UC.300A (final concentration 50nM) or negative control siRNA (Dharmacon Negative Control #1, final concentration 50 nM) using the transfection reagent Lipofectamine (Invitrogen). Media was changed after 24hrs. RNA was extracted 120hrs after transfection. Gene expression microarray analysis was carried out using Roche NimbleGens 4x72K Homo Sapiens gene expression array.

2012-10-14 | E-MEXP-3712 | biostudies-arrayexpress

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