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Endosymbiont bacteria in Arcella species (Amoebozoa, Arcellinida)

ABSTRACT: Endosymbiont bacteria in Arcella species (Amoebozoa, Arcellinida)

PROVIDER: PRJEB45379 | ENA |

REPOSITORIES: ENA

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Arcellinida

Project description:High diversity of testate amoebae (Amoebozoa, Arcellinida) detected by HTS analyses in a New England fen using newly-designed taxon-specific primers

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A needle in a haystack: a new metabarcoding approach to survey diversity at the species level of Arcellinida (Amoebozoa: Tubulinea)

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In Vitro Culture of the Insect Endosymbiont Spiroplasma poulsonii Highlights Bacterial Genes Involved in Host-Symbiont Interaction

Project description:Endosymbiotic bacteria associated with eukaryotic hosts are omnipresent in nature, particularly in insects. Studying the bacterial side of host-symbiont interactions is, however, often limited by the unculturability and genetic intractability of the symbionts. Spiroplasma poulsonii is a maternally transmitted bacterial endosymbiont that is naturally associated with several Drosophila species. S. poulsonii strongly affects its host’s physiology, for example by causing male killing or by protecting it against various parasites. Despite intense work on this model since the 1950s, attempts to cultivate endosymbiotic Spiroplasma in vitro have failed so far. Here, we developed a method to sustain the in vitro culture of S. poulsonii by optimizing a commercially accessible medium. We also provide a complete genome assembly, including the first sequence of a natural plasmid of an endosymbiotic Spiroplasma species. Last, by comparing the transcriptome of the in vitro culture to the transcriptome of bacteria extracted from the host, we identified genes putatively involved in host-symbiont interactions. This work provides new opportunities to study the physiology of endosymbiotic Spiroplasma and paves the way to dissect insect-endosymbiont interactions with two genetically tractable partners.

2018-03-24 | GSE112290 | GEO

On the morphology, biometry and biogeography of Lamtopyxiscallistoma (Amoebozoa: Arcellinida).

Project description:The ultra-structure of the shell and the morphometric variability of soil inhabiting testate amoeba Lamtopyxiscallistoma from Madagascar were studied by using light- and scanning electron microscopy. The biometrical characteristic of the species was made on the basis of 75 specimens measured. In addition to the diameter of the shell, six other shell characters were described biometrically for the first time. The analysis of the variation coefficients shows that the studied population of L.callistoma is comparatively homogeneous and almost all measured characters are weakly to moderate variable (CV less than 10%). Scanning electron microscopy (SEM) studies on the shell ultra-morphology show that it has a smooth apertural surface with a thick layer of porous and fibrous organic cement and a rough dorsal surface composed of bigger and angular pieces of quartz. The shell wall has a thickness of about 5-6 µm and is composed of three layers. Unlike the previously accepted opinion that species is characterized by the presence of four teeth, this study shows that population of L.callistoma from Madagascar is comprised of both, specimens with four teeth and specimens with three teeth, in ratio of about 60% to 40%. Taking into account the restricted geographical distribution, large sizes and characteristic apertural morphology of L.callistoma it is assumed that this species, like some bryophilic 'Nebelas' with circumaustral distribution (e.g. Apoderavas, Alocoderacockayni, Certesellacertesi, Certesellamartiali, etc.), can be used as an example that in free-living microbial eukaryotes 'not everything is everywhere'.

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Identification of cross-species microRNA-based Communication in Brugia malayi with its endosymbiont Wolbachia

Project description:Brugia malayi is a parasitic nematode that causes lymphatic filariasis in humans. A total of 178 novel microRNA were identified from short read transcriptional data, which when combined with known Brugia microRNAs yielded a total of 284 microRNA. Of these, 123 microRNA sequences (43%) are differentially expressed over the mammalian life stages of B. malayi that we examined. Putative targets of these microRNA were identified from inversely expressed target clusters that contain valid seed sequences for the corresponding microRNAs. The largest identified cluster is downregulated in adult females and enriched in zinc finger domains, helicase domains, and DNA binding domains suggesting this microRNA cluster may have regulatory control over a large proportion of adult female specific mRNA genes. MicroRNA-like molecules are identified as produced by the Wolbachia endosymbiont, providing evidence for direct nucleic acid-based interdomain communication between filarial nematodes and their bacterial obligate endosymbiont.

2022-01-15 | GSE143730 | GEO

Expression of Wigglesworthia glossinidia endosymbiont of Glossina morsitans morsitans genes

Project description:Transcriptome analysis of Wigglesworthia glossinidia endosymbiont derived from uninfected and infected samples at 3 time points (3, 10 and 20 days). Expression profiling by array - Wigglesworthia glossinidia endosymbiont of Glossina morsitans morsitans

2015-03-10 | GSE55931 | GEO

Expression of Wigglesworthia glossinidia endosymbiont of Glossina morsitans morsitans genes (control vs self-cured)

2015-07-01 | GSE55991 | GEO

Expression of Wigglesworthia glossinidia endosymbiont of Glossina morsitans morsitans genes (control vs self-cured)

Project description:Transcriptome analysis of Wigglesworthia glossinidia endosymbiont derived from control samples with or without parasite contact at 10 days. Expression profiling by array - Wigglesworthia glossinidia endosymbiont of Glossina morsitans morsitans RNAs are a mix of Wigglesworthia, Sodalis and glossina. RNAs were extracted from 8 samples including 2 conditions (with 4 replicates per condition).

2015-07-01 | E-GEOD-55991 | biostudies-arrayexpress

Enrichment of Wolbachia Endosymbiont Transcripts from Host Nematode RNA by Cappable-seq

Project description:We report the application of Cappable-seq to selectively enrich prokaryotic endosymbiont transcripts from mixed host-symbiont total RNA.

2017-01-05 | GSE93139 | GEO

Genomic characterization of Neoparamoeba pemaquidensis (Amoebozoa) and its kinetoplastid endosymbiont.

Project description:We have performed a genomic characterization of a kinetoplastid protist living within the amoebozoan Neoparamoeba pemaquidensis. The genome of this "Ichthyobodo-related organism" was found to be unexpectedly large, with at least 11 chromosomes between 1.0 and 3.5 Mbp and a total genome size of at least 25 Mbp.

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