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Methods to Study PAMP-Triggered Immunity in Brassica Species

ABSTRACT: Methods to Study PAMP-Triggered Immunity in Brassica Species

PROVIDER: PRJEB4549 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR338493.fastq.gz	Fastqsanger.gz
	ERR338494.fastq.gz	Fastqsanger.gz
	ERR338495.fastq.gz	Fastqsanger.gz
	ERR338496.fastq.gz	Fastqsanger.gz
	ERR338497.fastq.gz	Fastqsanger.gz

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Arabidopsis acton binding proteins WLIM2A links PAMP-triggered immunity and cytoskeletal organization

Project description:Arabidopsis acton binding proteins WLIM2A links PAMP-triggered immunity and cytoskeletal organization

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PAMP-triggered genetic reprogramming involves widespread alternative transcription initiation and an immediate transcription factor wave

Project description:Immune responses triggered by pathogen-associated molecular patterns (PAMPs) are key to pathogen defense, but drivers and stabilizers of the growth-to-defense genetic reprogramming remain incompletely understood in plants. Here, we report a time-course study of the establishment of PAMP-triggered immunity (PTI) using cap analysis of gene expression. We show that around 15% of all transcription start sites (TSSs) rapidly induced during PTI define alternative transcription initiation events. From these, we identify clear examples of regulatory TSS change via alternative inclusion of target peptides or domains in encoded proteins, or of upstream open reading frames in mRNA leader sequences. We also find that 60% of PAMP response genes respond earlier than previously thought. In particular, a cluster of rapidly and transiently PAMP-induced genes is enriched in transcription factors (TFs) whose functions, previously associated with biological processes as diverse as abiotic stress adaptation and stem cell activity, appear to converge on growth restriction. Furthermore, examples of known potentiators of PTI, in one case under direct mitogen-activated protein kinase control, support the notion that the rapidly induced TFs could constitute direct links to PTI signaling pathways and drive gene expression changes underlying establishment of the immune state.

2021-12-15 | GSE144356 | GEO

PAMP-triggered genetic reprogramming involves widespread alternative transcription initiation and an immediate transcription factor wave

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Arabidopsis acton binding proteins WLIM2A links PAMP-triggered immunity and cytoskeletal organization

Project description:MAPKs are a family of highly conserved serine/threonine protein kinases that link upstream receptors to their downstream targets which can be localized in the cytoplasm or the nucleus. Pathogens produce pathogen-associated molecular patterns (PAMPs) that trigger the activation of MAPK cascades in plants. Phosphoproteomic analysis of PAMP-induced Arabidopsis plants led to the identification of several putative MAPK targets. WLIM2A is a putative phosphorylation target of MAPKs, but so far little is known about the function of WLIM2A in plant immunity. We investigated the role of WLIM2A in immunity via a reverse-genetics approach generating wlim2a knockout lines using CRISPR-Cas9, as well as complementation and phosphosite mutated WLIM2A expression lines in the wlim2a background. The wlim2a lines were compromised in their response to Pst DC3000 but showed enhanced resistance to fungal infection by Botrytis cinereae. Transcriptome analyses revealed that immune hormone signaling and biosynthesis genes of salicylic acid (SA), jasmonic acid (JA) and ethylene (ET) are differentially regulated in the wlim2a knockout lines. Pathogen assays with Pst DC3000 showed altered stomatal phenotypes in wlim2a mutants. Importantly, WLIM2A phosphomutants had opposing stomatal behaviour and resistance phenotypes in response to Pst DC3000 infection. Overall, these data show that WLIM2A is a MAPK target and that phosphorylation of WLIM2A regulates stomatal immunity.

2024-11-14 | GSE236403 | GEO

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Quantitative phosphoproteomic analysis reveals common regulatory mechanisms of immune components during effector- and PAMP-triggered immunity

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Role of MPK4 in pathogen-associated molecular pattern-triggered alternative splicing in Arabidopsis

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