Project description:Verticillium dahliae Kleb., a soil-borne fungus that colonizes vascular tissues, induces wilting, chlorosis and early senescence in potato. Difference in senescence timing found in two diploid potato clones, 07506-01 and 12120-03, was studied and genetic variation in response to V. dahliae infection was identified as a causal factor. The clone, 07506-01, was infected with V. dahliae but did not develop symptoms, indicating tolerance to the pathogen. The other diploid clone, 12120-03 had low levels of pathogen with infection and moderate symptoms indicating partial resistance. 07506-01 was found to carry two susceptible alleles of the Ve2 gene and 12120-03 carried one Ve2 resistant and one susceptible allele. Infected leaves of the two clones were compared using gene expression profiling with the Potato Oligonucleotide Chip Initiative (POCI) microrarray. The results provide further evidence for differences in response of the two clones to infection with V. dahliae. Chlorophyll biosynthesis was higher in the tolerant 07506-01 compared to partially resistant 12120-03. On the other hand, expression of fungal defense genes, Ve resistance genes and defense phytohormone biosynthetic enzyme genes was decreased in 07506-01 compared to 12120-03 suggesting defense responses were suppressed in tolerance compared to resistance. Transcription factor gene expression differences pointed to the WRKY family as potential regulators of V. dahliae responses in potato. Two-color microarray comparison of two clones, three biological replicates of each clone, one dye swap technical replicate

2013-07-15 | E-GEOD-48881 | biostudies-arrayexpress

Single-cell RNA sequencing of human various osteosarcoma tissues

Project description:Single-cell RNA sequencing of human various osteosarcoma tissues

| PRJNA993663 | ENA

Comparison of foliage of diploid potato clones 07506-01 and 12120-03

2013-07-15 | GSE48881 | GEO

Acidovorax sp. 99 strain:99

Project description:Acidovorax sp. 99 transcriptome - Chisto_RNA_34

| PRJNA566720 | ENA

Gene expression profiles in RAW264.7 cells with CpG and Gf

Project description:RAW264.7 cells were untreated or treated with CpG+/-Gf for 6 hours. Total RNA was analyzed via Illumina Mouse Ref-8 V2. CpG‐induced genes and their regulation by Gf were examined.

2018-03-15 | GSE97366 | GEO

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