Project description:Death-promoting transcription factor Btf has been reportedly implicated in the apoptotic signal triggered by ionizing radiation, DNA damage-inducing agents, and cyclic stretch. However, mechanisms for the regulation of Btf-mediated apoptosis remain largely unknown. In the present study, we performed genome-wide mRNA expression profiling from Btf-depleted Hela cells and identified SMAD3 as a potential downstream target of Btf. Btf interacted with the SMAD3 promoter and this interaction was associated with the activation of SMAD3 gene transcription. Furthermore, we provided evidence that SMAD3 is involved in the induction of apoptosis, and that the disruption of Btf-mediated SMAD3 expression leads to resistance to DNA damage-induced apoptosis
Project description:Here we show that depletion of the homologous non-classical serine-arginine-rich (SR) splicing factors Btf (BCLAF1) and TRAP150 causes mitotic defects. We propose that, in addition to their previously reported roles in maintaining mRNA distribution, Btf and TRAP150 control abundance of transcripts encoding mitotic regulators thereby affecting mitotic progression in human cells.
Project description:In order to evaluate the identification of genes and pathways, the global gene expression profiles were assessed in response to three chemicals (benzene (B), toluene (T), formaldehyde (F)) individually and as a BTF mix on zebrafish. We performed whole genome DNA microarray experiments with subsequent quantitative analysis conducted on selected genes. We used synchronized zebrafish populations exposed to B, T, F or a BTF mix, and whole genome microarrays to screen for global changes in zebrafish transcription profiles. Young adults of zebrafish were selected for RNA extraction and hybridization on Affymetrix microarrays.
Project description:In order to evaluate the identification of genes and pathways, the global gene expression profiles were assessed in response to three chemicals (benzene (B), toluene (T), formaldehyde (F)) individually and as a BTF mix on the soil nematode, Caenorhabditis elegans. We performed whole genome DNA microarray experiments with subsequent quantitative analysis conducted on selected genes. We used synchronized C. elegans populations exposed to B, T, F or a BTF mix, and whole genome microarrays to screen for global changes in C. elegans transcription profiles. Young adults of C. elegans were selected for RNA extraction and hybridization on Affymetrix microarrays.
Project description:In order to evaluate the identification of genes and pathways, the global gene expression profiles were assessed in response to three chemicals (benzene (B), toluene (T), formaldehyde (F)) individually and as a BTF mix on zebrafish. We performed whole genome DNA microarray experiments with subsequent quantitative analysis conducted on selected genes.
Project description:In order to evaluate the identification of genes and pathways, the global gene expression profiles were assessed in response to three chemicals (benzene (B), toluene (T), formaldehyde (F)) individually and as a BTF mix on the soil nematode, Caenorhabditis elegans. We performed whole genome DNA microarray experiments with subsequent quantitative analysis conducted on selected genes.
