Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Cross-species screening platforms identify EPS-8 as a critical link for mitochondrial stress and actin stabilization.

ABSTRACT: Cross-species screening platforms identify EPS-8 as a critical link for mitochondrial stress and actin stabilization.

PROVIDER: PRJEB46972 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR6496860.fastq.gz	Fastqsanger.gz
	ERR6496870.fastq.gz	Fastqsanger.gz
	ERR6496861.fastq.gz	Fastqsanger.gz
	ERR6496871.fastq.gz	Fastqsanger.gz
	ERR6496862.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 18

Similar Datasets

Cross-species screening platforms identify EPS-8 as a critical link for mitochondrial stress and actin stabilization.

Project description:1) CRISPR-Cas9 screening of 3.5ng/mL rotenone-treated NPCs versus an ethanol control (treatments include 8 days and 23 days of treatment). 2) RNA-sequencing of NPCs treated with 10µg/mL antimycin A for 8 hours compared to an ethanol control 3) RNA-sequencing of whole C. elegans grown on empty-vector control or fed eps-8 RNAi

2021-12-01 | E-MTAB-10843 | biostudies-arrayexpress

Cross-species screening platforms identify EPS-8 as a critical link for mitochondrial stress and actin stabilization.

Project description:The dysfunction of mitochondria is associated with the physiological consequences of aging and many age-related diseases. Therefore, critical quality control mechanisms exist to protect mitochondrial functions, including the unfolded protein response of the mitochondria (UPRMT). However, it is still unclear how UPRMT is regulated in mammals with mechanistic discrepancies between previous studies. Here, we reasoned that a study of conserved mechanisms could provide a uniquely powerful way to reveal previously uncharacterized components of the mammalian UPRMT. We performed cross-species comparison of genetic requirements for survival under—and in response to—mitochondrial stress between karyotypically normal human stem cells and the nematode Caenorhabditis elegans. We identified a role for EPS-8/EPS8 (epidermal growth factor receptor pathway substrate 8), a signaling protein adaptor, in general mitochondrial homeostasis and UPRMT regulation through integrin-mediated remodeling of the actin cytoskeleton. This study also highlights the use of cross-species comparisons in genetic screens to interrogate cellular pathways.

| S-EPMC8555897 | biostudies-literature

Transcriptome analyses of in vitro exercise by EPS and adrenaline

Project description:To evaluate transcriptomic changes induced by in vitro exercise, we established two in vitro exercise models; EPS (electrical pulse stimulation and clenbuterol treatment). As for clen-buterol treatment, differentiated C2C12 myotubes were treated by 30 ng/ml clenbuterol for 1 hour and control and clenbuterol treated C2C12 myotubes were analyzed by RNA-sequencing. As for an EPS model, EPS was applied to differentiated C2C12 myotubes for 24 hours and control and EPS applied C2C12 myotubes were analyzed by RNA-sequencing.

2021-11-06 | GSE183159 | GEO

A long noncoding RNA lincRNA-EPS acts as a transcriptional brake to restrain inflammation

Project description:LincRNA-EPS is a long noncoding RNA that is expressed in macrophages and downregulated upon exposure to diverse microbial products. Analysis of macrophages from lincRNA-EPS-deficient mice revealed a specific role for this lincRNA in restraining immune response gene (IRG) expression. Mechanistically, lincRNA-EPS associates with chromatin at regulatory regions of IRGs to repress their transcription. To gain insight into the molecular function of lincRNA-EPS on chromatin, we generated ATAC-Seq libraries from wildtype and lincRNA-EPS-deficient macrophages stimulated with LPS.

2016-05-08 | GSE78873 | GEO

Gene expression changes induced in Rice (Oryza sativa) leaves following treatment with any one of the following: wild type Xoo, lipopolysaccharide (LPS) and exopolysaccaride (EPS) deficient Xoo mutant; LPS proficient but EPS deficient Xoo mutant

Project description:Lipopolysaccharide is a Microbe Associated Molecular Pattern (MAMP) that is known to induce defense responses in plants. In rice we have shown that Xoo LPS induce callose deposition, reactive oxygen production and induced resistance response. The exopolysaccaride (EPS) secreted by Xoo might be involved in supressing these defense responses. We have performed transcriptional profiling of rice leaf gene expression changes after treatment with Xoo strains BXO1003 (LPS-, EPS-), BXO1002 (LPS+ EPS-) and BXO43 (wild type) along with milliQ treated leaves to identify the genes that are differentially expressed. RNA was isolated from mid veins of rice leaves 15 hours after injecting them with Xoo strains BXO1003 (LPS-, EPS-), BXO1002 (EPS-), BXO43 (wild type) or milli-Q water. The rice gene expression in each of the treatment was normalized based on the gene expression in the milli-Q treatment.

2015-06-30 | E-GEOD-61833 | biostudies-arrayexpress

EPS model colon

Project description:EPS Bifidobacterium and model colon microbiota

| PRJEB35291 | ENA

Variovorax paradoxus EPS

Project description:Variovorax paradoxus EPS genome sequencing project

| PRJNA43457 | ENA

Bif EPS samples

Project description:Bif EPS samples Raw sequence reads

| PRJNA480119 | ENA

A NET4-RabG3 couple mediate the link between actin and the tonoplast and is essential for normal actin cytoskeletal remodelling in stomatal closure to flg22

Project description:Members of the NETWORKED (NET) family are involved in actin-membrane interactions. Here we show that two members of the NET family, NET4A and NET4B, are essential for normal guard cell actin reorganization, which is a process critical for stomatal closure in plant immunity. NET4 proteins interact with F-actin and with members of the Rab7 GTPase RABG3 family through two distinct domains, allowing for simultaneous localization to actin filaments and the tonoplast. NET4 proteins interact with GTP-bound, active RABG3 members, suggesting their function being downstream effectors. We also show that RABG3b is critical for stomatal closure induced by microbial patterns. Taken together, we conclude that the actin cytoskeletal remodelling during stomatal closure involves a molecular link between actin filaments and the tonoplast, which is mediated by the NET4-RABG3b interaction. We propose that stomatal closure to microbial patterns involves the coordinated action of immune-triggered osmotic changes and actin cytoskeletal remodelling likely driving compact vacuolar morphologies.

2023-09-22 | PXD044681 | Pride

Variovorax paradoxus EPS

Project description:Variovorax paradoxus EPS Transcriptome or Gene expression

| PRJNA594416 | ENA