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DrMedArab

ABSTRACT: Medicago arabica, genomic and transcriptomic data

PROVIDER: PRJEB47303 | ENA |

REPOSITORIES: ENA

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Dataset's files

Source:

			Action	DRS
	ERR6688404_1.fastq.gz	Fastqsanger.gz
	ERR6688404_2.fastq.gz	Fastqsanger.gz
	ERR6688725_1.fastq.gz	Fastqsanger.gz
	ERR6688725_2.fastq.gz	Fastqsanger.gz
	ERR6688726_1.fastq.gz	Fastqsanger.gz

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Overexpression of MtHSFA9 in hairy roots of Medicago truncatula

Project description:HSFA9 (Medtr4g126070) is a seed-specific heat shock transcription factor that is upregulated during the later stages of seed maturation. To identify genes that are regulated by this transcription factor in the model legume Medicago truncatula, Medicago hairy roots were generated using Agrobacterium rhizogenes transformed with the genomic sequence of the genomic HSFA9 gene of Medicago. Using the Medicago NimbleGen chip, a transciptomic analysis was performed to identify differentially expressed genes compared to the GUS expressed control

2020-05-20 | GSE150862 | GEO

Overexpression of MtABI3 in transgenic Medicago truncatula roots

Project description:ABI3 is a B3-domain transcription factor that acts as a master regulator of seed maturation. To identify genes that are regulated by this transcription factor in the model legume Medicago truncatula, Medicago hairy roots were generated using Agrobacterium rhizogenes transformed with the genomic sequence of the ABI3 gene of Medicago. Using the Medicago NimbleGen chip, a transciptomic analysis was performed to identify differentially expressed genes compared to the GUS expressed control.

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Overexpression of MtABI3 splicing forms by ectopically expressing cDNA sequences in transgenic Medicago truncatula hairy roots generated from an abi3 mutant background (mutant NF6003)

Project description:ABI3 is a B3-domain transcription factor that acts as a master regulator of seed maturation. To identify genes that are regulated specifically by different splicing forms in the model legume Medicago truncatula, Medicago hairy roots obtained from an abi3 mutant background (NF6003) were generated using Agrobacterium rhizogenes transformed with the three cDNA sequence of the ABI3 gene splicing forms of Medicago. Using the Medicago NimbleGen chip, a transcriptomic analysis was performed to identify differentially expressed genes compared to the GUS expressed control.

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