Project description:100mM EMS was added to mid-log phase Halobacterium NRC-1 cultures. After constant stress of EMS for 30 minutes, cultures were spun down and pellets were re-suspended in same volume of GN101 media. Cultures were then harvested by centrifugation and pellets were snap frozen on a dry-ice ethanol bath. Samples for RNA preparation were collected during recovery time points at 0, 10, 20, 30, 40, 60 and 120 minutes. Keywords: stress response, dose response
Project description:We performed DNA Adenine Methyltransferase Identification (DamID) to map the contacts between the genome and the nuclear lamina in the EMS lineage of early embryos. Dam::EMR-1 and GFP::Dam fusion proteins were expressed from single-copy transgenes inserted into an intergenic region on chromosome II. To induce a short burst of transcription of the transgenes, we used the med-1 promoter, which is active in the EMS cell of 4 cell-stage embryos and repressed in the descendants of EMS.
Project description:Granulosa cells are essential to the growth, development, and maturation of oocytes and follicles. In patients with EMs-related infertility, follicle maturation disorders, poor oocyte quality may be closely related to GCs in the follicle. However, the molecular biological mechanism of GCs in EMs-related infertility has not been reported. Therefore, we tried to explore the possible causes of EMs-related infertility through the study of ovarian GCs in patients with EMs-related infertility and tubal factor infertility.
Project description:100mM EMS was added to mid-log phase Halobacterium NRC-1 cultures. After constant stress of EMS for 30 minutes, cultures were spun down and pellets were re-suspended in same volume of GN101 media. Cultures were then harvested by centrifugation and pellets were snap frozen on a dry-ice ethanol bath. Samples for RNA preparation were collected during recovery time points at 0, 10, 20, 30, 40, 60 and 120 minutes. 16 samples (8 samples from EMS perturbed and 8 non-perturbed controls) were analyzed on replicate arrays (dye-flips) all against the same standard reference sample.
Project description:his experiment aims at analyzing crossover distribution genome-wide in the plant Arabidopis thaliana in response to varying patterns of polymorphism across all five chromosomes. In the publication (Madec et al, 2026), two different Recombinant Inbred Lines were used : 7RV168 and 7RV498, from a Columbia-0xCatania-1 (Col-0xCt-1) population (from Simon et al., 2008). Each were backcrossed to EMS treated Col-0 and Ct-1 to produce F1 plants. F1 plants were crossed as female or male to each other to produce F2 plants, which were sequenced. Refer to Figure 1 of Madec et al, 2026 for more information. In this specific population II, the F2 plants sequenced come from a cross between an F1 7RV168xCt-EMS as female and an F1 7RV168xCol-EMS as male. Other populations can be found linked to this submission
Project description:This experiment aims at analyzing crossover distribution genome-wide in the plant Arabidopis thaliana in response to varying patterns of polymorphism across all five chromosomes. In the publication (Madec et al, 2026), two different Recombinant Inbred Lines were used : 7RV168 and 7RV498, from a Columbia-0xCatania-1 (Col-0xCt-1) population (from Simon et al., 2008). Each were backcrossed to EMS treated Col-0 and Ct-1 to produce F1 plants. F1 plants were crossed as female or male to each other to produce F2 plants, which were sequenced. Refer to Figure 1 of Madec et al, 2026 for more information. In this specific population IV, the F2 plants sequenced come from a cross between an F1 7RV168xCt-EMS as female and an F1 7RV168xCol-EMS as male. Other populations can be found linked to this submission
Project description:This experiment aims at analyzing crossover distribution genome-wide in the plant Arabidopis thaliana in response to varying patterns of polymorphism across all five chromosomes. In the publication (Madec et al, 2026), two different Recombinant Inbred Lines were used : 7RV168 and 7RV498, from a Columbia-0xCatania-1 (Col-0xCt-1) population (from Simon et al., 2008). Each were backcrossed to EMS treated Col-0 and Ct-1 to produce F1 plants. F1 plants were crossed as female or male to each other to produce F2 plants, which were sequenced. Refer to Figure 1 of Madec et al, 2026 for more information. In this specific population III, the F2 plants sequenced come from a cross between an F1 7RV168xCol-EMS as female and an F1 7RV168xCt-EMS as male. Other populations can be found linked to this submission