Project description:Gas hydrates, also known as clathrates, are cages of ice-like water crystals encasing gas molecules such as methane (CH4). Despite the global importance of gas hydrates, their microbiomes remain mysterious. Microbial cells are physically associated with hydrates, and the taxonomy of these hydrate-associated microbiomes is distinct from non-hydrate-bearing sites. Global 16S rRNA gene surveys show that members of sub-clade JS-1 of the uncultivated bacterial candidate phylum Atribacteria are the dominant taxa in gas hydrates. The Atribacteria phylogeny is highly diverse, suggesting the potential for wide functional variation and niche specialization. Here, we examined the distribution, phylogeny, and metabolic potential of uncultivated Atribacteria in cold, salty, and high-pressure sediments beneath Hydrate Ridge, off the coast of Oregon, USA, using a combination of 16S rRNA gene amplicon, metagenomic, and metaproteomic analysis. Methods were developed to extract bacterial cellular protein from these sediments, as outlined below. Sample Description Three sediments samples were collected from beneath Hydrate Ridge, off the coast of Oregon, USA. Sediments were cored at ODP site 1244 (44°35.1784´N; 125°7.1902´W; 895 m water depth) on the eastern flank of Hydrate Ridge ~3 km northeast of the southern summit on ODP Leg 204 in 2002 and stored at -80°C at the IODP Gulf Coast Repository. E10H5 sediment is from 68.5 meters below sediment surface interface C1H2 sediment is from 2 meters below sediment surface interface. C3H4 sediment is from 21 meters below sediment surface interface.
2020-01-22 | PXD012479 | Pride
Project description:EMG produced TPA metagenomics assembly of PRJNA339844 data set (subsurface metagenome Metagenome).
| PRJEB41334 | ENA
Project description:EMG produced TPA metagenomics assembly of PRJNA390944 data set (Functional gene diversity and expression in methane-hydrate bearing deep subsurface sediments).
| PRJEB47803 | ENA
Project description:EMG produced TPA metagenomics assembly of PRJNA264715 data set (Costa Rica margin subsurface Metagenome).
| PRJEB51544 | ENA
Project description:EMG produced TPA metagenomics assembly of PRJEB30246 data set (Metagenomic_analysis_nasopharyngeal_colonisation).
| PRJEB75197 | ENA
Project description:EMG produced TPA metagenomics assembly of PRJNA639280 data set (v).
| PRJEB72785 | ENA
Project description:EMG produced TPA metagenomics assembly of PRJNA986291 data set (CForBio.metagenome).
| PRJEB71756 | ENA
Project description:EMG produced TPA metagenomics assembly of PRJNA757683 data set (Apical Periodontitis).
| PRJEB56742 | ENA
Project description:EMG produced TPA metagenomics assembly of PRJNA785116 data set (Metagenomic sequencing).