Project description:Burkholderia mallei and Burkholderia pseudomallei are both potential biological threats agents. Melioidosis caused by B. pseudomallei is endemic in Southeast Asia and Northern Australia, while glanders caused by B. mallei infections are rare. Here we studied the proteomes of different B. mallei and B. pseudomallei isolates to determine species specific characteristics. Analyzing the expressed proteomes of B. mallei and B. pseudomallei revealed differences between B. mallei and B. pseudomallei but also between isolates from the same species. Expression of multiple virulence factors and proteins of several PKS/NRPS clusters was demonstrated. Proteome analysis can be used not only to identify bacteria but also to characterize the expression of important factors that putatively contribute to pathogenesis of B. mallei and B. pseudomallei.
Project description:To use whole genome microarrays to compare the differences in genome contents of 5 B. pseudomallei isolated from clinical specimens and environmental sample with B. pseudomallei K96243 reference strain and reveals variable patterns of Genomic Islands (GIs) Keywords: Comparative genomic hybridization DNA microarrays were used to compare genome of clinical and environmental B. pseudomallei isolates with B. pseudomallei K96243 reference strain (B. pseudomallei K96243 vs. B. pseudmallei isolates). Each hybridization was used for comparison between B. pseudomallei K96243 as a reference strain with environmental isolate BP45s, environmental isolate BP28L, clinical isolate H307, clinical isolate P54, clinical isolate P82. Two replicate per array. Multiple hits with 90-99.99 % identity correspond to other locus are replicate of their genes were averaged and analyzed.
Project description:We used the microarray data to analyze host cells response on A549 cells infected with A/duck/Malaysia/F189/07/2004(H5N2) A/duck/Malaysia/F189/07/2004(H5N2) infected A549 cells were harvested at 10 hpi and RNA extraction was performed using standard protocol as described by Affymetrix. The aim of this experiment is to analyze host response to Influenza A/duck/Malaysia/F189/07/2004(H5N2) infection.
Project description:We used the microarray data to analyze host cells response on A549 cells infected with A/duck/Malaysia/F119/3/1997(H5N3) A/duck/Malaysia/F119/3/1997(H5N3) infected A549 cells were harvested at 10 hpi and RNA extraction was performed using standard protocol as described by Affymetrix. The aim of this experiment is to analyze host response to Influenza A/duck/Malaysia/F119/3/1997(H5N3) infection.
Project description:We used the microarray data to analyze host cells response on CEF cells infected with A/duck/Malaysia/F59/04/1998(H5N2) A/duck/Malaysia/F59/04/1998(H5N2) infected CEF cells were harvested at 10 hpi and RNA extraction was performed using standard protocol as described by Affymetrix. The aim of this experiment is to analyze host response to Influenza A/duck/Malaysia/F59/04/1998(H5N2) infection.
Project description:We used the microarray data to analyze host cells response on MDCK cells infected with A/duck/Malaysia/F189/07/2004(H5N2) A/duck/Malaysia/F189/07/2004(H5N2) infected MDCK cells were harvested at 10 hpi and RNA extraction was performed using standard protocol as described by Affymetrix. The aim of this experiment is to analyze host response to Influenza A/duck/Malaysia/F189/07/2004(H5N2) infection.
Project description:We used the microarray data to analyze host cells response on A549 cells infected with A/duck/Malaysia/F59/04/1998(H5N2) A/duck/Malaysia/F59/04/1998(H5N2) infected A549 cells were harvested at 10 hpi and RNA extraction was performed using standard protocol as described by Affymetrix. The aim of this experiment is to analyze host response to Influenza A/duck/Malaysia/F59/04/1998(H5N2) infection.
Project description:We used the microarray data to analyze host cells response on CEF cells infected with A/duck/Malaysia/F189/07/2004(H5N2) A/duck/Malaysia/F189/07/2004(H5N2) infected CEF cells were harvested at 10 hpi and RNA extraction was performed using standard protocol as described by Affymetrix. The aim of this experiment is to analyze host response to Influenza A/duck/Malaysia/F189/07/2004(H5N2) infection.
Project description:We used the microarray data to analyze host cells response on MDCK cells infected with A/duck/Malaysia/F119/3/1997(H5N3) A/duck/Malaysia/F119/3/1997(H5N3) infected MDCK cells were harvested at 10 hpi and RNA extraction was performed using standard protocol as described by Affymetrix. The aim of this experiment is to analyze host response to Influenza A/duck/Malaysia/F119/3/1997(H5N3) infection.
