Project description:Genome editing was conducted on a t(3;8) K562 model to investigate the effects of deleting different modules or CTCF binding sites within the MYC super-enhancer. To check mutations after targeting with CRISPR-Cas9 we performed amplicon sequencing using the Illumina PCR-based custom amplicon sequencing method using the TruSeq Custom Amplicon index kit (Illumina). The first PCR was performed using Q5 polymerase (NEB), the second nested PCR with KAPA HiFi HotStart Ready mix (Roche). Samples were sequenced paired-end (2x 250bp) on a MiSeq (Illumina).

2021-07-26 | E-MTAB-9937 | biostudies-arrayexpress

KACST_test

Project description:the Ecoli fastq file

| PRJEB13962 | ENA

peat metagenome

Project description:Illumina MiSeq paired end sequencing

| PRJDB12674 | ENA

SRiD- A facile DNA barcode generation and management system for high throughput screening

Project description:20 random DNA barcodes were designed in silico and transfected into PC3 cells. Barcodes were sequenced using Illumina-Miseq technology to find the sequence and their respective copy numbers. Current file contains the raw data of these DNA barcodes in fastq format Validating an algorithm called SRiD that generates random DNA barcodes that do not match a genome of interest, in this case human genome. 20 DNA barcodes were used for this validation.

2016-03-31 | E-GEOD-67441 | biostudies-arrayexpress

Project description:the fastq file of Illumina and Nanopore for lung biopsy tissue Metagenome

| PRJNA744136 | ENA

Project description:the fastq file of Illumina and Nanopore for lung biopsy tissue Metagenome

| PRJNA744341 | ENA

The influence of nt-LNA on cell composition and phenotypees in the atherosclerotic lesion.

Project description:Raw fastq files from paired-end, Illumina-based sequencing on NovaSeq6000

2021-11-01 | GSE160791 | GEO

High-throughput RNA Sequencing of THP-1 macrophages treated with or without native LDL for 24h.

Project description:Raw fastq files from paired-end, Illumina-based sequencing on NovaSeq6000

2021-11-01 | GSE160615 | GEO