Project description:We present a draft genome assembly that includes 200 Gb of Illumina reads, 4 Gb of Moleculo synthetic long-reads and 108 Gb of Chicago libraries, with a final size matching the estimated genome size of 2.7 Gb, and a scaffold N50 of 4.8 Mb. We also present an alternative assembly including 27 Gb raw reads generated using the Pacific Biosciences platform. In addition, we sequenced the proteome of the same individual and RNA from three different tissue types from three other species of squid species (Onychoteuthis banksii, Dosidicus gigas, and Sthenoteuthis oualaniensis) to assist genome annotation. We annotated 33,406 protein coding genes supported by evidence and the genome completeness estimated by BUSCO reached 92%. Repetitive regions cover 49.17% of the genome.
Project description:Streptomyces bingchenggensis is a soil bacterium that produces a family of macrolide antibiotics, milbemycins, which is commercially important in crop protection, human and veterinary medicine. After the complete genome sequence, and annotation, for further development of our gene expression approach to biosynthesis, we have employed whole genome microarray expression profiling as a discovery platform to obtain improved specificity and sensitivity of gene expression analysis, allowing a global and at the same time detailed picture of how gene clusters for secondary metabolism are modulated. In the result, we confirmed the expression mil and nan gene cluster, furthermore, pks3, pks5 and nrps7, nrps8 also showed significant gene expression, but no obvious products detected. In Streptomyces bingchenggensis, there are also corresponding genes belonging to Defense mechanisms, which is much more than other Streptomyces, for the resistance of own metabolites and dealing with complex environmental factors.
Project description:The draft genome of L. sativa (lettuce) cv. Tizian was sequenced in two Illumina sequencing runs, mate pair and shotgun. This entry contains the RAW sequencing data.
Project description:This study compared the genome of Streptomyces rimosus rimosus against that of Streptomyces coelicolor. It also compared 4 strains with changes in oxytetracycline production and derived from G7, the type strain, against G7. Keywords: Comparative genomic hybridization