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JaNemVect

ABSTRACT: Nematostella vectensis, genomic and transcriptomic data

PROVIDER: PRJEB50784 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR10123674_1.fastq.gz	Fastqsanger.gz
	ERR10123674_2.fastq.gz	Fastqsanger.gz
	ERR8575398.fastq.gz	Fastqsanger.gz
	ERR8571699_1.fastq.gz	Fastqsanger.gz
	ERR8571699_2.fastq.gz	Fastqsanger.gz

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Evolutionary conservation of the eumetazoan gene regulatory landscape

Project description:Bilaterian animals differ from other metazoans in their apparent bilateral symmetry and the development of a third germ layer. Both might have facilitated the evolution of the diverse and complex bilaterian body plans. The first cnidarian genome sequence revealed that despite their morphological simplicity, this sister group to all bilaterians shares an immense genomic complexity with vertebrates. This suggested that it might have been the complexity of gene regulation which increased during the evolution of bilaterians. We compared the gene regulatory landscape of cnidarians and bilaterians. To this end we generated the first genome-wide prediction of gene regulatory elements and profiled five epigenetic marks in a non-bilaterian animal, the cnidarian Nematostella vectensis. We found that the location of chromatin modifications relative to genes and distal enhancers is conserved among eumetazoans. Surprisingly, the genomic landscape of gene regulatory elements is highly similar between Nematostella and bilaterian model organisms. This suggests that complex regulation of developmental gene expression evolved in eumetazoans without a major increase in complexity in bilaterians. ChIP-seq of p300, RNA Pol2, and five histone modifications in Nematostella vectensis.

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Geographical temperature stress in the sea anemone Nematostella vectensis

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Project description:We used bacteria isolated from field samples of Nematostella vectensis to quantify gene expression through comparisons of transcriptomes.

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jaNemVect

Project description:Nematostella vectensis

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ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics

Project description:We performed single-cell transcriptome analysis (using 10x genomics 3' scRNA-seq v3.1 chemistry) in the sea anemone Nematostella vectensis (Cnidaria).

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Project description:We characterised the genome organization in non-bilaterian animals (sponge Ephydatia muelleri, ctenophores Mnemiopsis leidyi and Hormiphora californensis, placozoans Trichoplax adhaerens and Cladtertia collaboinventa, and cnidarian Nematostella vectensis) and close unicellular relatives (ichthyosporeans Sphaeroforma arctica, filasterean Capsaspora owczarzaki and choanoflagellate Salpingoeca rosetta) by combining high-resolution chromosome conformation capture (Micro-C) with profiling of chromatin marks (ChIP-seq), accessible genomic regions (ATAC-seq) and gene expression (MARS-seq).

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Chromatin loops are an ancestral hallmark of the animal regulatory genome [ATAC-seq]

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Decoupling behavioral and transcriptional responses to color in an eyeless cnidarian

Project description:We utilized the eyeless sea anemone, Nematostella vectensis, to quantify gene expression differences between different colors of light (red, green, blue) and in constant darkness through comparisons of 96 transcriptomes

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