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WGS genome of strain STM 5069

ABSTRACT: Genome sequence of Mesorhizobium escarrei STM 5069T

PROVIDER: PRJEB51177 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	ERR9354955_1.fastq.gz	Fastqsanger.gz
	ERR9354955_2.fastq.gz	Fastqsanger.gz

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Effect of STm-delta-aroA on CD4+ T cell activation

Project description:The experiment was designed to test how tumour conditoned media from STm-delta-aroA infected tumour organoids would modulate T cell activation. Using splenocytes from Nr4a3-Tocky Great Smart-17A mice, T cells were activated with soluble anti-CD3 and anti-CD28 antibodies. At 4 and 16 h live CD4+ T cells were FACS sorted and RNA extracted. Analysis revealed that T cells exposed to STm-delta-aroA conditioned tumor medium failed to upregulate metabolic pathways associated with canonical T cell activation.

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