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Slamseq of whole mouse primary cortical neurons (mPCN) with perturbed mRNA degradation machinery

ABSTRACT: Slamseq of whole mouse primary cortical neurons (mPCN) with perturbed mRNA degradation machinery

PROVIDER: PRJEB51784 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR9322000.fastq.gz	Fastqsanger.gz
	ERR9322010.fastq.gz	Fastqsanger.gz
	ERR9322020.fastq.gz	Fastqsanger.gz
	ERR9322001.fastq.gz	Fastqsanger.gz
	ERR9322011.fastq.gz	Fastqsanger.gz

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Slamseq of whole mouse primary cortical neurons (mPCN) with perturbed mRNA degradation machinery

Project description:We perturbed mRNA degradation machinery in mouse primary cortical neurons (mPCN) and investigated the change in mRNA half-lives. We performed SLAMseq Metabolic RNA Labeling on Mutant mPCN line harbouring a ponasteroneA-inducible heterozygous dominant-negative Caf1 (dnCaf1) and on GFP-transfected mPCNs. The Slamseq technique provided snapshots of mRNA kinetics allowing to estimate mRNA half-lives.

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