Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

PolyI:C stimulation of KO vs. WT BMDMs

ABSTRACT: PolyI:C stimulation of KO vs. WT BMDMs

PROVIDER: PRJEB5257 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR414138.fastq.gz	Fastqsanger.gz
	ERR414139.fastq.gz	Fastqsanger.gz
	ERR414140.fastq.gz	Fastqsanger.gz
	ERR414141.fastq.gz	Fastqsanger.gz
	ERR414142.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 18

Similar Datasets

PolyI:C stimulation of KO vs. WT BMDMs

Project description:Primary murine bone marrow derived macrophages (BMDMs) of Setdb2GT/GT and WT mice were stimulated with polyI:C and harvested at 0, 2 and 8h. Everything with biological triplicates (=cells from 3 individual mice per condition)

2014-11-23 | E-MTAB-2263 | biostudies-arrayexpress

RNA-seq data of TAGAP WT or KO BMDMs treated with Curdlan

Project description:Bone marrow derived macrophages (BMDMs) were isolated form WT or TAGAP-KO mice, followed by stimulation with Curdlan (10ug/ml) for 0 or 3 hours, and samples were collected and sent to BGI for RNA SEQ analysis.

2020-03-02 | GSE146156 | GEO

The proteomic of IL-4 treated BMDMs from Wt and Elp3 KO mice

Project description:This experiement aims to know what the differences of protein translation are in the bone marrow derived macro-phages(BMDMs) from WT mice and Elp3 KO mice. We treated the BMDMs with or without IL-4 for 4 hours.

2022-09-12 | PXD033962 | Pride

LPS time-course in WT and Stk40 KO BMDMs

Project description:Bone marrow was harvested from Rosa26CreER; Stk40+/+ (WT; n = 3) and Rosa26CreER; Stk40loxp/loxp (Stk40 KO; n = 3) mice and differentiated for 6 days in the presence of 100 nM 4-OHT to generate WT and Stk40 KO bone-marrow derived macrophages (BMDMs). 2. On day 7 following differentiation BMDMs were treated with 100 ng x ml-1 LPS and harvested at 0 hrs, 6 hrs, 16 hrs, and 32 hrs following LPS exposure. 3. The cells were snap-frozen at the time of harvest. RNA was extracted using the Qiagen RNeasy mini kit as per manufacturer’s protocol including the on-column DNase digestion. Groups: There are cells from 3 mice x 2 genotypes x 4 time points G1: WT 0 hr LPS G2: WT 6 hr LPS G3: WT 16 hr LPS G4: WT 32 hr LPS G5: Stk40 KO 0 hr LPS G6: Stk40 KO 6 hr LPS G7: Stk40 KO 16 hr LPS G8: Stk40 KO 32 hr LPS

2018-12-28 | GSE124410 | GEO

Kinetics of LPS induction in wt and Cop1-ko BMDMs

Project description:Bone marrow was extracted from mice that are COP1-wt Rosa26-CreERT2 or COP1-floxed Rosa26-CreERT2 BMDMs were obtained by culturing bone marrow precursors in media containing 20% of supernatant from L929 cells. At day 4 of differentiation 4-OHT was added at 1uM to induce deletion of COP1 in BMDMs derived from COP1-floxed mice. At day 7 of differentiation, BMDMs were treated with 100 ng/ml of LPS or not. BMDMs were directly harvested in lysis buffer (from Qiagen RNeasy mini kit) at different time points (0h, 2.5h, 2.5h, 4h, 6h, 9h and 13h) following LPS stimulation. Three BMDMs preparations per group: G1: BMDMs from COP1-wt mice (expressing the wt allele of COP1) CRE positive. G2: BMDMs from COP1-floxed mice (expressing the floxed allele of COP1) CRE positive

2018-10-03 | GSE114762 | GEO

Expression data from monocyte-derived macrophages after stimulation with mock, LPS, PolyI:C and P3C.

Project description:Pre-stimulation of MDMs with LPS (signals via MyD88 and TRIF dependent pathways) and PolyI:C (signals via a TRIF dependent pathway) leads to a reduced viral infection. In contrast, pre-stimulation with P3C (signals via MyD88 dependent pathway) does not lead to a reduced viral infection. This microarray was performed to find genes that are specifically upregulated in LPS and PolyI:C stimulated MDMs but not P3C stimulated MDMs. So to give us leads into the mechanism involved in the reduction of viral infection. MDMs of four different donors were stimulated for 4h with mock, LPS, PolyI:C or P3C. RNA was isolated and gene expression of these cells was assessed. Gene expression of LPS, PolyI:C and P3C stimulated MDMs was compared to mock.

2013-07-01 | E-GEOD-41295 | biostudies-arrayexpress

Expression data from monocyte-derived macrophages after stimulation with mock, LPS, PolyI:C and P3C.

2013-07-01 | GSE41295 | GEO

Transcript profiling of WT and SIRPα KO BMDMs post-M1 activation in the presence of mCD47.ex

Project description:To compare the inflammatory responses of WT and SIRPα KO macrophage, we performed a complete transcript profiling of WT and SIRPα-KO M1 macrophage using transcriptome sequencing as a discovery platform. SIRPα-KO mice and WT mice were kept under the same condition. BMDMs were produced from WT and SIRPα-KO mice followed by M1 polarization. RNA was then isolated from the same number of BMDMs.

2021-03-19 | GSE169177 | GEO

Bone marrow derived macrophages RNA-Seq (WT vs SIRPa KO)

Project description:RNA transcriptome difference between WT and SIRPa knockout (KO) bone marrow derived macrophages (BMDMs). To understand how SIRPa inhibits the phagocytotic capacity of macrophages, a mouse lacking SIRPa was generated, and the transcriptional profiles of BMDMs from wild-type and SIRPa KO mice were compared, using RNA sequencing.

2023-10-02 | GSE223059 | GEO

LPS time-course in WT and Stk40 KO BMDMs

Project description:LPS time-course in WT and Stk40 KO BMDMs

| PRJNA511979 | ENA