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Plasmodium knowlesi Population Genetics

ABSTRACT: Population genetic analysis of Plasmodium knowlesi reveals differential selection and exchange events between Borneo and Peninsular sub-populations

PROVIDER: PRJEB52783 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	ERR9751930_1.fastq.gz	Fastqsanger.gz
	ERR9751930_2.fastq.gz	Fastqsanger.gz
	ERR9751940_1.fastq.gz	Fastqsanger.gz
	ERR9751940_2.fastq.gz	Fastqsanger.gz
	ERR9751950_1.fastq.gz	Fastqsanger.gz

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Project description:The purpose of this reasearch is to identify and evaluate the global gene expression of Plasmodium knowlesi blood-stage parasites and specifically compare the gene expression profiles of samples derived from ex vivo versus long-term in vitro cultures. For ex vivo samples, a rhesus monkey was infected with P. knowlesi to obtain ring iRBCs to establish a short-term culture to immediately collect ex vivo derived time points every 4 hours in the course of the parasite’s 24-hour life cycle. For in vivo samples, they were generated from the same clone in the ex vivo culture adapted to long-term in vitro culture and were collected every 4 hours in the course of the parasite's 24-hour life cycle. Two replicate experiments(Aa and Ha) were developed from in vitro cultures. Samples labeled with Cy5 were hybridized against a reference RNA pool labeled with Cy3, consisting of equal amounts of P. knowlesi RNA from each time point.

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