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ATAC seq for L3.6pl cells: Parental and resistant to paclitaxel [PacR]

ABSTRACT: ATAC seq for L3.6pl cells: Parental and resistant to paclitaxel [PacR]

PROVIDER: PRJEB52808 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	ERR9723870_1.fastq.gz	Fastqsanger.gz
	ERR9723870_2.fastq.gz	Fastqsanger.gz
	ERR9723867_1.fastq.gz	Fastqsanger.gz
	ERR9723867_2.fastq.gz	Fastqsanger.gz
	ERR9723868_1.fastq.gz	Fastqsanger.gz

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Project description:Acquired drug resistance represents a major challenge in chemo-therapy treatment for various types of cancers. We have found that the retinoid X receptor–selective agonist bexarotene (LGD1069, Targretin) was efficacious in treating chemo-resistant cancer cells. The goal of this microarray study was to understand the mechanism of bexarotene’s role in overcoming acquired drug resistance using human breast cancer cells MDA-MB-231 as a model system and paclitaxel as model compound. After MDA-MB-231 cells were repeatedly treated with paclitaxel for 8 cycles with each cycle including a 3-day treatment with 30 nM paclitaxel and followed by a 7-day exposure to control medium, MDA cells resistant to paclitaxel were developed and their growth was no longer inhibited by paclitaxel treatment. Those MDA cells with acquired drug resistance, when treated with paclitaxel and bexarotene in combination, could regain their sensitivity and their growth were again inhibited. Therefore, RNA samples from parental MDA-MB-231 cells, paclitaxel-resistant MDA cells treated with vehicle, paclitaxel alone or in combination with bexarotene, were used for perform global gene expression profiling with Affymetrix HG-U133A gene chips. Keywords: Drug Treatment

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