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ChIP-seq of H3K27ac and H3K4me3 in HAP1 tRNA gene locus modified clones and control clones

ABSTRACT: ChIP-seq of H3K27ac and H3K4me3 in HAP1 tRNA gene locus modified clones and control clones

PROVIDER: PRJEB53804 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR9873810.fastq.gz	Fastqsanger.gz
	ERR9873811.fastq.gz	Fastqsanger.gz
	ERR9873812.fastq.gz	Fastqsanger.gz
	ERR9873813.fastq.gz	Fastqsanger.gz
	ERR9873814.fastq.gz	Fastqsanger.gz

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ChIP-seq of H3K27ac and H3K4me3 in HAP1 tRNA gene locus modified clones and control clones

Project description:This dataset contains ChIP-seq data profiling genomic binding of H3K27ac and H3K4me3 in single cell-derived control, as well as CRISPR/Cas9 induced tRNA gene deletion clones and intergenic region deletion clones in human cancer cell lines HAP1. In this study, we found a large genomic deletion of 10q23 in Cas9 modified clones and further investigate the effect of H3K27ac binding.

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Project description:This dataset contains ChIP-seq data of H3K4me3 and Pol III in single cell-derived control and CRISPR/Cas9 induced tRNA gene deletion clones in human cancer cell lines HAP1 and HepG2. In this study, we looked into functional Cas9-induced on-target genomic alteration in our tRNA gene deletion clones, HAP1 t72 and HepG2 t15.

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ChIP-seq of Pol III and H3K4me3 in HAP1 and HepG2 tRNA gene deletion clones and control clones

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Project description:Purpose: The aim of the experiment was to eludicate differentialy expressed genes (DEGs) upon treatment with alkylating agent MMS between WT and MED13 deficient HAP1 cells. Results: After bioinformatic processing, at ≥2-fold change and an FDR≤0.1, 446 DEGs were identified in MED13 KO cells when compared to WT. Upon the MMS treatment 394 DEGs were identified in MED13 KO cells, of which 229 were common with untreated MED13 KO cells. Conclusions: Common DEGs represented genes directly regulated by MED13.

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