Project description:To investigate the heterogeneity of lung stromal cells and identify the specific lung stromal subset, we performed single cell RNA-sequencing (scRNA-seq) on lung stromal cells (CD45-CD31-CD326-). Around 6800 cells were captured using the 10x Chromium technology.
Project description:These samples, isolated from untransformed marrow stromal cells from wild-type C57Bl/6 mice, serve as a control set. The cells (CD31-, CD34 low/-, CD45-) are from a mixed population of 100% bone marrow stromal cells arising after more than two months in culture and 10-15 passages. Keywords: other
Project description:These samples, isolated from untransformed marrow stromal cells from wild-type C57Bl/6 mice, serve as a control set. The cells (CD31-, CD34 low/-, CD45-) are from a mixed population of 100% bone marrow stromal cells arising after more than two months in culture and 10-15 passages. Experiment Overall Design: this experiment include 1 samples and 9 replicates
Project description:We report the RNAseq data obtained from 50.000-100.000 CD31-/CD45- pneumocytes isolated by FACS from mice harboring a normal dose or one extra copy of the Sirt1 gene, and a tamoxifen-inducible oncogenic KI alelle of KRasG12V after 4 weeks of tamoxifen treatment. Pneumocytes with the activated form of the inducible KRasG12V oncogene sere selected making use of the reporter gene LacZ (located next to the oncogene in the same polycistronic mRNA), by loading CD31-/CD45- pneumocytes with the LacZ-activated fuorogenic molecule FDG prior to FACS sorting.
Project description:We report the first time of the transcriptome difference between IL35- and PBS-injected ischemic muscle after hindlimb ischemia, specifically in isolated CD45-CD31+ endothelial cell
Project description:To understand if cancer cells that metastasize to the lung induce changes of gene expression in associated endothelial cells to promote metastatic growth, we profiled the gene expression of CD31+/CD45- cells sorted from unchallenged normal mouse lungs and dissected metastatic nodules.
Project description:The objective of this study was to understand the mechanisms by which electrical stimulation in vivo leads to improvements and growth in skeletal muscle post exercise through changing the function of CD146+CD45-CD31- pericytes. We utilized global gene profiling to identify novel signaling pathways and patterns of gene expression involved in this adaptation process.