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RNAseq of murine fetal liver cells overexpressing hnRNP K

ABSTRACT: RNAseq of murine fetal liver cells overexpressing hnRNP K

PROVIDER: PRJEB53947 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR9883770_1.fastq.gz	Fastqsanger.gz
	ERR9883770_2.fastq.gz	Fastqsanger.gz
	ERR9883771_1.fastq.gz	Fastqsanger.gz
	ERR9883771_2.fastq.gz	Fastqsanger.gz
	ERR9883772_1.fastq.gz	Fastqsanger.gz

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RNAseq of murine fetal liver cells overexpressing hnRNP K

Project description:Background: hnRNP K is an RNA-binding protein that's been implicated in oncogenesis, particularly in hematological disorders. Intent: To determine the impact of hnRNP K overexpression on RNA expression in murine fetal liver cells. Experimental Workflow: Fetal liver cells were subjected to retrovial transduction with either empty vector GFP or hnRNP K GFP. Purified GFP+ cells were flow sorted, and RNA was extracted from the GFP+ population, upon which RNA sequencing was performed.

2023-06-10 | E-MTAB-11886 | biostudies-arrayexpress

Analysis of Fetal liver cells from hnRNP L KO mice

Project description:The regulation of post-transcriptional modifications of pre-mRNA by alternative splicing is important for cellular function, development and immunity. To investigate the role of hnRNP L in this process, we have generated conditional hnRNP L knockout mice carrying floxed alleles in order to generate a specific defect in hnRNP L activity. We have previously characterized defects present in the thymocytes of hnRNP L -/- mice and the current study extends the analysis to examine the effects in fetal liver cells. Overall these cells exhibit a very different phenotype from thymocytes characterized by significant cell death and a potential block in proliferation. Further functional studies were performed to better characterize these effects. RNA-seq from fetal liver cells of WT mice compared to KO (hnRNP L) mice

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Analysis of Fetal liver cells from hnRNP L KO mice

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mRNA expression of murine hematopoietic stem cells and ex vivo murine MLL-AF9 cells deficient for total PRDM16 or f-PRDM16, and AF9 cells overexpressing individual PRDM16 isoforms

Project description:Group 1 -- WT or PRDM16-KO ex vivo murine MLL-AF9 cells, and PRDM16-KO AF9 cells overexpressing either f-PRDM16 or s-PRDM16. Group 2 -- WT or total PRDM16-KO murine HSCs isolated from adult BM. Group 3 -- WT or total PRDM16-KO murine HSCs isolated from fetal liver. Group 4 -- WT or f-PRDM16-KO murine HSCs (expressing s-PRDM16 only) isolated from fetal liver.

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Single cell RNAseq analysis of murine fetal growth plate chondrocytes and perichondrial cells

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Proteomic analysis of laser-microdissected murine fetal glomeruli

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RNAseq of human Multiple Sclerosis cortex with hnRNP A1 dysfunction

Project description:RNAseq of human Multiple Sclerosis cortex with hnRNP A1 dysfunction

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Integrative genome-wide analysis reveals cooperative regulation of alternative splicing by hnRNP proteins (RNA-Seq)

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