RNAseq of murine fetal liver cells overexpressing hnRNP K
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB53947 | ENA |
REPOSITORIES: ENA
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Project description:Background: hnRNP K is an RNA-binding protein that's been implicated in oncogenesis, particularly in hematological disorders. Intent: To determine the impact of hnRNP K overexpression on RNA expression in murine fetal liver cells. Experimental Workflow: Fetal liver cells were subjected to retrovial transduction with either empty vector GFP or hnRNP K GFP. Purified GFP+ cells were flow sorted, and RNA was extracted from the GFP+ population, upon which RNA sequencing was performed.
2023-06-10 | E-MTAB-11886 | biostudies-arrayexpress
Project description:The regulation of post-transcriptional modifications of pre-mRNA by alternative splicing is important for cellular function, development and immunity. To investigate the role of hnRNP L in this process, we have generated conditional hnRNP L knockout mice carrying floxed alleles in order to generate a specific defect in hnRNP L activity. We have previously characterized defects present in the thymocytes of hnRNP L -/- mice and the current study extends the analysis to examine the effects in fetal liver cells. Overall these cells exhibit a very different phenotype from thymocytes characterized by significant cell death and a potential block in proliferation. Further functional studies were performed to better characterize these effects. RNA-seq from fetal liver cells of WT mice compared to KO (hnRNP L) mice
2013-12-31 | E-GEOD-33815 | biostudies-arrayexpress
Project description:The regulation of post-transcriptional modifications of pre-mRNA by alternative splicing is important for cellular function, development and immunity. To investigate the role of hnRNP L in this process, we have generated conditional hnRNP L knockout mice carrying floxed alleles in order to generate a specific defect in hnRNP L activity. We have previously characterized defects present in the thymocytes of hnRNP L -/- mice and the current study extends the analysis to examine the effects in fetal liver cells. Overall these cells exhibit a very different phenotype from thymocytes characterized by significant cell death and a potential block in proliferation. Further functional studies were performed to better characterize these effects.
2013-12-31 | GSE33815 | GEO
Project description:Hematopoiesis in Murine Fancd2-/- vs WT Fetal Liver
| PRJNA727546 | ENA
Project description:Group 1 -- WT or PRDM16-KO ex vivo murine MLL-AF9 cells, and PRDM16-KO AF9 cells overexpressing either f-PRDM16 or s-PRDM16. Group 2 -- WT or total PRDM16-KO murine HSCs isolated from adult BM. Group 3 -- WT or total PRDM16-KO murine HSCs isolated from fetal liver. Group 4 -- WT or f-PRDM16-KO murine HSCs (expressing s-PRDM16 only) isolated from fetal liver.
2018-06-08 | GSE112860 | GEO
Project description:We sought to identify alterations in RNA expression and splicing in human grey matter from the brains of people with Multiple Sclerosis (MS) where we observe mislocalization of the RNA binding protein hnRNP A1. Thus, RNA was extracted from fresh-frozen post-mortem brain samples of three people with MS and compared to RNA extracted from fresh-frozen post-mortem brain samples of three healthy controls by RNAseq. Regions of interest in human grey matter were defined by immunostaining for hnRNP A1 to confirm grey matter regions with hnRNP A1 mislocalization in MS tissue, and grey matter regions with normal hnRNP A1 localization in control tissue, and the same region from an adjacent tissue section was dissected with a razor blade and used for RNA extraction and sequencing.
2024-01-04 | GSE207680 | GEO
Project description:Alteration of fetal kidney morphogenesis in diabetic pregnancies is poorly described, especially changes in the extracellular matrix (ECM) and glomerular basement membrane (GBM) during glomerulogenesis. Addressing the ECM proteome, or matrisome, in mouse fetal glomeruli in a healthy and diabetic environment will improve understanding about the association between ECM and GBM changes in the glomerulus as potential reprogramming mechanisms for glomerular dysfunction in infants of mothers with diabetes. This project aimed to define the matrisome and BM composition in the maturing murine fetal glomeruli collected from normal and diabetic mothers. For this, we used laser microdissection microscopy to isolate maturing glomeruli from cryosections, and analyse by label-free tandem mass spectrometry to test the hypothesis that maternal diabetes influences ECM and GBM composition, assembly and function.
2021-10-13 | PXD025911 | Pride
Project description:The proliferation and survival of hematopoietic stem cells (HSCs) has to be strictly coordinated to ensure the timely production of all blood cells. Here we report that the splice factor and RNA binding protein hnRNP L (heterogeneous nuclear ribonucleoprotein L) is required for hematopoiesis, since its genetic ablation in mice reduces almost all blood cell lineages and causes premature death of the animals. In agreement with this, we observed that hnRNP L deficient HSCs lack both the ability to self-renew and foster hematopoietic differentiation in transplanted hosts. They also display mitochondrial dysfunction, elevated levels of γH2AX, are Annexin V positive and incorporate propidium iodide indicating that they undergo cell death. Lin(-)c-Kit(+) fetal liver cells from hnRNP L deficient mice show high p53 protein levels and up-regulation of p53 target genes. In addition, cells lacking hnRNP L up-regulated the expression of the death receptors TrailR2 and CD95/Fas and show Caspase-3, Caspase-8 and Parp cleavage. Treatment with the pan-caspase inhibitor Z-VAD-fmk, but not the deletion of p53, restored cell survival in hnRNP L deficient cells. Our data suggest that hnRNP L is critical for the survival and functional integrity of HSCs by restricting the activation of caspase-dependent death receptor pathways.
2016-06-07 | GSE57875 | GEO
Project description:We isolated murine fetal liver and murine adult bone marrow and FACS sorted LT-HSCs, ST-HSCs and MPPs. We used global expression analysis by microarray to compare regulated genesets in different HSC populations in fetal and adult.
2023-04-30 | GSE173158 | GEO
Project description:The aim of this study was to analyze the transcriptome of TER119+ fetal liver cells in the absence of the transcription factor KLF3 at murine embryonic day E14.5
2014-02-23 | GSE50554 | GEO