Project description:Phagocytosis is a conserved cellular mechanism for food uptake, defense and general animal-microbe interactions in metazoans. How the discrimination and subsequent processing of native and foreign microbes is facilitated remains largely unknown in marine invertebrates. Thereto, we combined a recently developed phagocytic assay with proteomics analysis to compare the phagocytic activity of the sponge Halichondria panicea upon encounter with a native (i.e., isolated from H. panicea; Hal 281) and a foreign (i.e., isolated from Nematostella vectensis; NJ1) Vibrio isolate.

2025-05-27 | PXD063400 | Pride

Transcriptomics Analysis of the Impacts of Exposure to WAF, CEWAF and BaP on sponge Halichondria panicea (phylum Porifera)

Project description:Exposure of sponge Halichondria panicea to hydrocarbon and dispersant contaminated seawater

| PRJEB38335 | ENA

Metatranscriptomics analysis of Halichondria panicea exposed to hydrocarbons

Project description:Metatranscriptomics analysis of sponge Halichondria panicea exposed to marine snow, marine oil snow and dispersed oil contaminated seawater.

| PRJEB49864 | ENA

Comprehensive investigation of marine Actinobacteria associated with the sponge Halichondria panicea.

Project description:Representatives of Actinobacteria were isolated from the marine sponge Halichondria panicea collected from the Baltic Sea (Germany). For the first time, a comprehensive investigation was performed with regard to phylogenetic strain identification, secondary metabolite profiling, bioactivity determination, and genetic exploration of biosynthetic genes, especially concerning the relationships of the abundance of biosynthesis gene fragments to the number and diversity of produced secondary metabolites. All strains were phylogenetically identified by 16S rRNA gene sequence analyses and were found to belong to the genera Actinoalloteichus, Micrococcus, Micromonospora, Nocardiopsis, and Streptomyces. Secondary metabolite profiles of 46 actinobacterial strains were evaluated, 122 different substances were identified, and 88 so far unidentified compounds were detected. The extracts from most of the cultures showed biological activities. In addition, the presence of biosynthesis genes encoding polyketide synthases (PKSs) and nonribosomal peptide synthetases (NRPSs) in 30 strains was established. It was shown that strains in which either PKS or NRPS genes were identified produced a significantly higher number of metabolites and exhibited a larger number of unidentified, possibly new metabolites than other strains. Therefore, the presence of PKS and NRPS genes is a good indicator for the selection of strains to isolate new natural products.

| S-EPMC2876447 | biostudies-literature

Co-cultivation of the marine sponge Halichondria panicea and its associated microorganisms.

Project description:Marine sponges host bacterial symbionts with biotechnological potential, yet isolation of true sponge symbionts remains difficult due to their host dependency. Moreover, attempts to grow sponges for their pharmacologically-active compounds outside of their habitat often results in a shift of their microbial community. In this study we evaluate suitable sponge cultivation methods that allow maintenance of both the marine sponge Halichondria panicea and its associated bacteria in an ex situ environment. In addition, we present a method for co-cultivation of sponge explants and microbes separated by a membrane in a multi-chamber device. Tests on ex situ cultivation of H. panicea under different controlled conditions showed that only high water exchange rates in the aquarium enabled maintenance of its dominant symbiont "Candidatus Halichondribacter symbioticus" at a high relative abundance in the sponge body, a prerequisite for co-cultivation. The bacterial enrichment retrieved from co-cultivation contained bacteria from nine different classes in addition to sequences corresponding to "Ca. H. symbioticus". This represents an increase of the cultivable bacterial classes from H. panicea compared to standard isolation techniques on solid media plates. The current study provides insights into sponge-microbe maintenance under ex situ conditions and proposes a new method for the isolation of sponge-associated bacteria.

| S-EPMC6639338 | biostudies-literature

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