Project description:To investigate the influence of transcription factor knockouts in cell fate decision-making, we performed a CROP-seq screen of 20 transcription factors in brain organoids.
Project description:Organoids were generated from H9 cells. Single cells were sorted from 4-month-old brain organoids differentiated using the telencephalon organoids protocol.
Project description:To study the effect of GLI3 knockout on early brain organoid development, we collected single-cell multiome data from 18 day old brain organoids
Project description:Single-cell RNA sequencing of 4-month-old telencephalon organoids. Organoids were generated from cells infected with pooled lentivirus library. The lentiviral library delivers dual-gRNAs targeting 36 high-risk ASD genes in parallel. Organoids were dissociated after 4 months of in vitro differentiation and maturation. Single cells were sorted and subjected to 10X genomics 3' kits.