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RNA-seq data U2OS human osteosarcoma cell line etoposide treated with JMY knockdown

ABSTRACT: RNA-seq data U2OS human osteosarcoma cell line etoposide treated with JMY knockdown

PROVIDER: PRJEB55290 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	ERR10040863_1.fastq.gz	Fastqsanger.gz
	ERR10040863_2.fastq.gz	Fastqsanger.gz
	ERR10040864_1.fastq.gz	Fastqsanger.gz
	ERR10040864_2.fastq.gz	Fastqsanger.gz

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RNA-seq data U2OS human osteosarcoma cell line etoposide treated with JMY knockdown

Project description:Effects of JMY on gene expression in U2OS under etoposide treatment

2023-04-20 | E-MTAB-12059 | biostudies-arrayexpress

Whole-genome CRISPR screen of etoposide-treated U2OS and U2OS p53KO cells, and RNAseq of U2OS cells incubated in media conditioned by etoposide-treated U2OS or U2OS p53KO cells.

Project description:CRISPR screen: U2OS or U2OS p53KO cells expressing Cas9 were transduced with a whole-genome library of CRISPR sgRNAs, then treated with either DMSO or etoposide. Differential sgRNA abundances were calculated for each condition and used to determine the effect of each single gene knockout on fitness and the drug-induced death rate. RNA-Seq: U2OS or U2OS p53KO cells were cultured with either DMSO or etoposide for 48 hours, and then U2OS cells were incubated in this conditioned media for 8 hours. RNA was collected and use to observe differential expression changes between conditions.

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U2OS osteosarcoma cells LC-MSMS

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Whole-genome CRISPR screen of etoposide-treated U2OS and U2OS p53KO cells, and RNAseq of U2OS cells incubated in media conditioned by etoposide-treated U2OS or U2OS p53KO cells.

Project description:Whole-genome CRISPR screen of etoposide-treated U2OS and U2OS p53KO cells, and RNAseq of U2OS cells incubated in media conditioned by etoposide-treated U2OS or U2OS p53KO cells.

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Gene regulation by m6A demethylase ALKBH5 in U2OS osteosarcoma

Project description:To identify target genes regulated by ALKBH5 in osteosarcoma, we silenced the expression of ALKBH5 in osteosarcoma cell line-U2OS and tested its effect on U2OS transcriptome.

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Project description:In our study, we found that SQLE was critical to osteosarcoma progression. To identify the mechanism of SQLE in osteosarcoma, we conducted the RNA-seq analysis of SQLE knockdown in osteosarcoma cell line U2OS cells.

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mRNA-seq for etoposide-treated TLP-knockdown cells

Project description:mRNA-seq for etoposide-treated TLP-knockdown cells

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