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Identification of adaptive mechanisms leading to reduced antibiotic susceptibility in bacterial biofilms using experimental evolution. Part 2: Burkholderia cenocepacia strain J2315 biofilms on beads

ABSTRACT: Identification of adaptive mechanisms leading to reduced antibiotic susceptibility in bacterial biofilms using experimental evolution. Part 2: Burkholderia cenocepacia strain J2315 biofilms on beads

PROVIDER: PRJEB55418 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	ERR10087443.fastq.gz	Fastqsanger.gz
	ERR10087445.fastq.gz	Fastqsanger.gz
	ERR10087446.fastq.gz	Fastqsanger.gz
	ERR10087448.fastq.gz	Fastqsanger.gz

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Identification of adaptive mechanisms leading to reduced antibiotic susceptibility in bacterial biofilms using experimental evolution. Part 2: Burkholderia cenocepacia strain J2315 biofilms on beads

Project description:Burkholderia cenocepacia J2315 was repeatedly and intermittently exposed to tobramycin, ciprofloxacin or meropenem. Bacteria were grown on cryobeads submerged in liquid BHI medium. After 24 hours, the beads were washed and fresh medium with of without antibiotics added. After another 24 hours of incubation, the beads were washed, the bacteria removed from the beads, and used for inoculation of fresh beads. This was repeated to a total of up to ten cycles. Evolved lineages were then DNA-sequenced to screen for genome changes.

2022-08-18 | E-MTAB-12092 | biostudies-arrayexpress

Expression data from Burkholderia cenocepacia J2315 hfq and hfq2 mutants

2014-01-01 | GSE47344 | GEO

Burkholderia cenocepacia J2315

Project description:Burkholderia cenocepacia J2315 Genome sequencing

| PRJNA326479 | ENA

Identification of potential therapeutic targets for Burkholderia cenocepacia by comparative transcriptomics

Project description:[1] Transcription profiling of one Burkholderia cenocepacia clinical isolate, J2315, versus a soil isolate, HI2424, in conditions mimicking CF sputum [2] Transcription profiling of Burkholderia cenocepacia isolates J2315 and HI2424 in media mimicking CF sputum or the soil environment

2009-12-01 | GSE15817 | GEO

Expression data from Burkholderia cenocepacia J2315 hfq and hfq2 mutants

Project description:Hfq proteins are RNA chaperones that play a critical role in post-transcription regulation of gene expression. Bacteria of the Burkholderia cepacia complex harbor two distinct and functional Hfq proteins, the Hfq and Hfq2. We have previously performed the functional analysis of Hfq and Hfq2 in the pathogen Burkholderia cenocepacia J2315. In order to examine the impacts of each RNA chaperone on the global transcriptome of B. cenocepacia J2315, we performed comparative transcriptome profile of mutants on the hfq and hfq2 genes, using as reference the wild-type strain. For expression profiling, over-night cultures of the Burkholderia cenocepacia J2315 wild-type strain and the isogenic mutants hfq::Tp and M-NM-^Thfq2 grown in LB medium were diluted to an initial OD640 nm of 0.25 into LB medium. Triplicate samples were cultured at 37M-BM-:C with 250 r.p.m. agitation for 16 h and RNA extracted from the three bacterial isolates.

2014-01-01 | E-GEOD-47344 | biostudies-arrayexpress

Gene expression in sRNA deletion mutant Burkholderia cenocepacia J2315 delta ncS35

Project description:The small RNA ncS35 was deleted from the Burkholderia cenocepacia J2315 genome. Gene expression of mutant was compared to wild type in three growth conditions: exponential phase and stationary phase planktonic growth and biofilm growth, all in LB medium.

2017-08-19 | E-MTAB-5526 | biostudies-arrayexpress

Response of Burkholderia cenocepacia J2315 to subinhibitory concentration of chlorhexidine

Project description:Burkholderia cenocepacia J2315 was grown in LB broth with and without the presence of the desinfectant chlorhexidine in the subinhibitory concentration of 5mg/L. Four biological replicates for each control and test conditions were performed. The experiment was designed as a direct comparison with dye swap of two replicates.

2012-05-10 | E-MEXP-3207 | biostudies-arrayexpress

Transcription profiling by array of Burkholderia cenocepacia J2315 grown at 20C or 37C

Project description:B. cenocepacia J2315 was grown on LB medium to mid-stationary phase at O.D. 0.5 at 150 rpm in a shaking incubator at two different temperatures: 37 degrees and 20 degrees centigrade.

2012-01-01 | E-MEXP-2789 | biostudies-arrayexpress

Transcription profiling of Burkholderia cenocepacia J2315 sessile cells to treatment with high doses of hydrogen peroxide, sodium hypochlorite and chlorhexidine

Project description:In order to unravel the molecular mechanisms involved in the resistance of biofilm-grown Burkholderia cenocepacia cells against high concentrations of disinfectants, the present study focussed on the transcriptional response in sessile B. cenocepacia J2315 cells following exposure to high levels of H2O2, NaOCl or chlorhexidine. In addition differences in gene expression were examined between untreated B. cenocepacia sessile cells and B. cenocepacia planktonic cells.

2010-03-02 | E-MEXP-2502 | biostudies-arrayexpress

Transcription profiling by array of Burkholderia cenocepacia J2315 wild type and antibiotic-resistant mutants exposed to sub-inhibitory concentrations of drugs

Project description:Burkholderia cenocepacia J2315 wild type and adaptive mutants with elevated resistance to antibiotics were exposed to sub-inhibitory concentrations of drugs

2010-08-01 | E-MEXP-2738 | biostudies-arrayexpress

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