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Glioblastoma RNAseq in TTFields-treated samples

ABSTRACT: TTFields Glioblastoma

PROVIDER: PRJEB56095 | ENA |

REPOSITORIES: ENA

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TTFields treatment effects on newly diagnosed Glioblastoma patients in peripheral T lymphocytes

Project description:We report the application of RNA-based sequencing technology for high-throughput profiling of T cell enriched peripheral blood mononuclear cells. By sequencing in total of 12 pairs GBM patient samples, we extracted TCRαβ V(D)J sequences from the deep RNA-seq of T cells isolated from the 24 PBMCs to determine if TTFields treatment affected TCR diversity, using the Simpson’s diversity index (DI), which is the average proportional abundance of TCR clones based on the weighted arithmetic mean. Of the 12 patients, 9 exhibited negative log fold change (logFC) of TCR DI after TTFields, indicating clonal expansion. Notably, in all but 1 patient, the top 200 most abundant clones post TTFields, which accounted for 38.1% to 100% (median 67%) of detectable clones, showed substantial expansion compared to pre-TTFields T cells, and inversely correlated with the DI. Thus, TTFields treatment is associated with adaptive immune activation as evidenced by clonal expansion of peripheral T cells.

2022-04-20 | GSE193729 | GEO

TTFields treatment effects on newly diagnosed Glioblastoma patients in peripheral T lymphocytes

Project description:TTFields treatment effects on newly diagnosed Glioblastoma patients in peripheral T lymphocytes

| PRJNA797422 | ENA

Monitor the dynamic changes of GBM cell lines under TTFields treatment

Project description:We used 3 established GBM cell lines to generate TTFields-resistant cells using Inovitro system.

2024-10-08 | GSE270513 | GEO

TTFields plus TMZ treatment effects on newly diagnosed Glioblastoma patients in peripheral blood

Project description:We report the application of single-cell-RNA-based sequencing technology for high-throughput profiling of peripheral blood mononuclear cells. By sequencing in total of 193,760 PBMCs in 12 GBM patients, we generated an overview of patient peripheral immune status with 38 biologically recongnized cell subtypes. By paired comparing pre- and post- treatment samples, we find a robust post-TTFields activation of adaptive immunity via the T1IFN trajectory anchored by plasmacytoid DCs. This study provides a framework for the application of scRNAseq towards characterization of peripheral immune activation status.

2025-01-01 | GSE193525 | GEO

TTFields plus TMZ treatment effects on newly diagnosed Glioblastoma patients in peripheral blood

Project description:TTFields plus TMZ treatment effects on newly diagnosed Glioblastoma patients in peripheral blood

| PRJNA796536 | ENA

RNAseq Analysis in glioblastoma cells treated with Mepazine

Project description:Glioblastoma cells were treated with vehicle (DMSO) or mepazine (MPZ, 20 μM, 4 hours) and prepared for RNAseq according to ActiveMotif standard procedure. The list of differentially expressed genes from DESeq2 output was selected based on 10% adjusted p-value level and FDR of 0.1.

2019-10-18 | GSE139018 | GEO

Gene expression analysis of electrical therapies on glioblastoma cell lines

Project description:The scarcity of effective treatment options for high grade brain tumours has led to a wide ranging search for alternative means of therapy for these difficult to treat tumours. Electrical field therapy is one such area that has been considered. The OptuneTM system is an FDA approved novel anti-mitotic device that delivers continuous alternating electric fields to the patient for the treatment of primary and recurrent Glioblastoma multiforme (GBM) (tumor treating fields - TTFields). Alternative electric fields delivery systems are also being investigated for the treatment of various cancers.To further explore alternative potential mechanisms of electric fields as a whole, we ran Optune, DBS electric fields treated and control untreated KNS42, U87 and GIN-31 (primary) cell lines on Clariom S Human Assays to produce genome-wide expression data.

2020-10-01 | E-MTAB-9043 | biostudies-arrayexpress

RNAseq Analysis in glioblastoma cells treated with Mepazine

Project description:RNAseq Analysis in glioblastoma cells treated with Mepazine

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Glioblastoma stem cells RNAseq profiling

Project description:RNAseq-profile of independent glioblastoma stem cells lines was determined

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Affymetrix array of three pancreatic cancer cells treated with tumour treating fields upon combination with mild hyperthermia

Project description:Tumor Treating Fields (TTFields) disturbs mitosis and consequently leads to cell cycle arrest and cell death. Mild hyperthermia induces cancer cell death by apoptosis,leading to DNA damage and disturbing DNA repair. Thus, when mild hyperthermia is combined with TTFields, the anti-tumor effect could be augmented. This prompted the hypothesis for the present thesis: TTFields and mild hyperthermia as synergistic modalities in Pancreatic ductal adenocarcinoma (PDAC) treatment could probably enhance antitumor efficacy and abate individual toxic effects through distinct and overlapping mechanisms target the tumor cell. Our established PDAC cell line (Bx-GEM) was treated with TTFields and TTFields with mild hyperthermia and was examined by microarray. 500 ng mRNA was checked for quality control and the concentration was measured again. Subsequently, gene expression profiling was performed using human HuGene-2_0-st-type array from Affymetrix.

2021-03-17 | E-MTAB-10270 | biostudies-arrayexpress

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