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Transcriptome profiling of tdTomato+ CGNPs from cerebellum of Math1-CreERT2 transgenic mice

ABSTRACT: Transcriptome profiling of tdTomato+ CGNPs from cerebellum of Math1-CreERT2 transgenic mice

PROVIDER: PRJEB56282 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR10286169.fastq.gz	Fastqsanger.gz
	ERR10286170.fastq.gz	Fastqsanger.gz
	ERR10286171.fastq.gz	Fastqsanger.gz
	ERR10286172.fastq.gz	Fastqsanger.gz
	ERR10286173.fastq.gz	Fastqsanger.gz

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Transcriptome profiling of tdTomato+ CGNPs from cerebellum of Math1-CreERT2 transgenic mice

Project description:Fluorescent cerebella were subsequently dissected at E15.5, E17.5, P0 (day of birth), P7 (postnatal day 7), P14 and P30; and tdTomato+ CGNPs were purified using fluorescence activated cell sorting (FACS). From these samples, we generated transcriptomes using RNA-seq

2022-09-25 | E-MTAB-7399 | biostudies-arrayexpress

RNA sequencing data of microglia isolated from brains of WT, Hexb-tdTomato, Hexb-CreERT2 and Hexb-KO mice. {Reporter]

Project description:RNA sequencing of FACS sorted microglia isolated from brain tissue of WT, Hexb-tdTomato, Hexb-CreERT2 and Hexb-KO mice was performed to test for potential differences in gene expression caused by the knock-in of tdTomato and CreERT2 into the Hexb locus.

2020-05-25 | GSE148412 | GEO

RNA sequencing data of microglia isolated from brains of WT, Hexb-tdTomato, Hexb-CreERT2 and Hexb-KO mice. [Cre]

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RNA sequencing data of microglia isolated from brains of WT, Hexb-tdTomato, Hexb-CreERT2 and Hexb-KO mice. [Hexb-KO]

2020-05-25 | GSE148411 | GEO

High throughput quantitative whole transcriptome analysis of CC10- B4+ and Krt5-CreERT2 - labeled distal lung cells

Project description:We used microfluidic single cell RNA-seq on adult isolated CC10-CreERT2 (negative) integrin beta4(pos) cells lung epithelial cells in order to determine the transcriptional profile of this putative progenitor population. CC10-CreERT2 / tdTomato (negative) integrin beta4(pos) cells were isolated by FACS, as were Krt5-CreERT2 / tdTomato (positive) cells. These cells were pooled and loaded onto the Fluidigm C1 device.

2014-11-17 | E-GEOD-61300 | biostudies-arrayexpress

scRNA-seq of Achilles tendons from Prg4-CreERT2 R26-tdTomato ATP mice

Project description:scRNA-seq of Achilles tendons from Prg4-CreERT2 R26-tdTomato ATP mice

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RNA Sequencing of postnatal mouse Math1-Cre; Sufu flox/- cerebellum

Project description:Transcriptional profiling to identify genes differentially regulated by Sufu during cerebellum development. Sufu was specifically deleted from the granule neuron compartment using Math1-driven Cre recombinase.

2020-01-15 | GSE143671 | GEO

The scRNA-seq of cells from Ren1c-/- Myh11-CreERT2 tdTomato Ren1c-YFP mice

Project description:The scRNA-seq of cells from Ren1c-/- Myh11-CreERT2 tdTomato Ren1c-YFP mice

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“Identifying the SLC25A46 interactome in transgenic mice: cerebellum”

Project description:SLC25A46 is an outer mitochondrial protein, member of the mitochondrial carrier family (SLC25), whereas mutations result in mitochondrial structure abnormalities leading to a wide spectrum of neurological disorders. SLC25A46 is known to interact with members of the MICOS (Mitochondrial Contact Site) complex involved in mitochondrial cristae organization, and components of the endoplasmic reticulum membrane. In the present work, our aim was to identify the interactome of SLC25A46 in cerebrum, cerebellum, heart and thymus from TgSLC25A46-FLAG transgenic mice through immunoprecipitation with anti-FLAG beads and proteomic analysis with LC-MS/MS.

2022-01-11 | PXD027599 | Pride

Smartseq3 Single-cell RNA-seq analysis of acutely FACS-purified tdTomato+ astrocyte derived cells from aged injured mice in vivo

Project description:To investigate the mechanisms that underlie astrocyte dedifferentiation, we performed single cell RNA sequencing analysis of tdTomato+ astrocyte derived cells approx 1 year after stab-wound injury. 2-3 month old GFAP-CreERT2;p53flox/flox;LSL-tdTomato (p53Gfap-icKO) mice were subjected to intracortical endoxifen injection to enable deletion of p53 gene and simultaneous expression of tdTomato for fate-mapping specifically in astrocytes (GFAP+) in the context of a stab-wound injury. GFAP-CreERT2;p53wt/wt;LSL-tdTomato (p53Gfapwt/wt) mice were used as control whereby p53 remains fate-mapped tdTomato+ astrocytes remain p53 wildtype after endoxifen injection. Approx 10-12 months after injury, mice were sacrificed and the peri-wound region was dissected. Mice of the same genotypes were pooled (3 p53Gfap-icKO and 2 p53Gfapwt/wt) and digested using the Miltenyi Adult brain dissociation kit. Single cell suspension was FACS sorted for tdTomato+ cells into 384 well plates and then Smartseq3 libraries were generated.

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