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Environmental detection of vector larvae

ABSTRACT: Towards environmental detection, quantification, and molecular characterization of Anopheles stephensi and Aedes aegypti from larval breeding sites

PROVIDER: PRJEB56471 | ENA |

REPOSITORIES: ENA

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Environmental detection of vector larvae

Project description:Towards environmental detection, quantification, and molecular characterization of Anopheles stephensi and Aedes aegypti from larval breeding sites

| PRJEB56414 | ENA

Eggs of the mosquito Aedes aegypti survive desiccation by rewiring their polyamine and lipid metabolism

Project description:Upon water loss, some organisms pause their life cycles and escape death. While widespread in microbes, this is less common in animals. Aedes mosquitoes are vectors for viral diseases. Aedes eggs can survive dry environments, but molecular and cellular principles enabling egg survival through desiccation remain unknown. In this report, we find that Aedes aegypti eggs, in contrast to Anopheles stephensi, survive desiccation by acquiring desiccation tolerance at a late developmental stage. We uncover unique proteome and metabolic state changes in Aedes embryos during desiccation that reflect reduced central carbon metabolism, rewiring towards polyamine production, and enhanced lipid utilization for energy and polyamine synthesis. Using inhibitors targeting these processes in blood-fed mosquitoes that lay eggs, we infer a two-step process of desiccation tolerance in Aedes eggs. The metabolic rewiring towards lipid breakdown and dependent polyamine accumulation confers resistance to desiccation. Furthermore, rapid lipid breakdown is required to fuel energetic requirements upon water re-entry to enable larval hatching and survival upon rehydration. This study is fundamental to understanding Aedes embryo survival and in controlling the spread of these mosquitoes.

2023-09-26 | PXD044525 | Pride

Identification of molecular mechanisms associated with resistance to the neonicotinoid insecticide imidacloprid in the dengue vector Aedes aegypti

Project description:This microarray study aimed at comparing constitutive gene expression levels between an Aedes aegypti insecticide-resistant strain (Imida-R) selected at the larval stage with the neonicotinoid insecticide imidacloprid for 10 generations and the parental strain (Bora-Bora) susceptible to all insecticides. Strains comparison was performed at both larval (4th stage larvae) and adult (3 days-old adults females, non-blood fed) stages.

2012-03-31 | E-MTAB-616 | biostudies-arrayexpress

Multifaceted Functional Implications of an Endogenously Expressed tRNA Fragment in Vector Mosquito Aedes aegypti

Project description:We performed small RNA sequencing (Illumina MiSeq) of mosquito Aedes aegypti to profile expression of tRNA derived fragments.

2018-01-03 | GSE101956 | GEO

Aedes aegypti aegypti strain:UGAL

Project description:Aedes aegypti aegypti larval transcriptome reads

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Aedes aegypti sex specific gene expression

Project description:This analysis compare gene expression between 4 day old sugar fed female and male Aedes aegypti mosquitoes. Keywords: Aedes aegypti sex specific expression

2007-05-15 | GSE7813 | GEO

Expression analysis of Meiotic drive system in Aedes aegypti during spermatogenesis

Project description:Investigation of whole genome gene expression level changes of testes in the meiotic drive system in aedes aegypti during spermatogenesis compared to non drive strain. The meiotic drive system in Aedes aegypti causes the female determining chromosome to fragment during spermatogenesis. A six chip study using total RNA from three separately extracted non driving strain testes of Aedes aegypti and three separately extracted meiotic drive strain testes of Aedes aegypti.

2010-07-21 | E-GEOD-23039 | biostudies-arrayexpress

Transcription profiling of Aedes aegypti 4th stage-larvae to various xenobiotics, including insecticides, polycyclic aromatic hydrocarbons, herbicides and heavy metals

Project description:This microarray experiment aimed at studying the response of Aedes aegypti 4th stage-larvae to various xenobiotics, including insecticides, polycyclic aromatic hydrocarbons, herbicides and heavy metals.

2008-12-31 | E-TABM-353 | biostudies-arrayexpress

Expression analysis of Meiotic drive system in Aedes aegypti during spermatogenesis

2010-07-21 | GSE23039 | GEO

Post-transcriptional microRNA modifications in the Dengue mosquito vector, Aedes aegypti

Project description:MicroRNAs (miRNA) have alternative forms known as isomiRs, which differ from each other by a few nucleotides. Next generation sequencing platforms facilitate identification of these isomiRs and recent discoveries regarding their functional importance have increased our understandings of the regulatory complexities of the microRNAome. Observed changes in the miRNA profiles in mosquitoes infected with flaviviruses have implicated small RNAs in the interactions between viruses and their vectors. Here we analysed the isomiR profiles of both uninfected and infected blood fed Aedes aegypti mosquitoes with a major human pathogen, Dengue virus at two time points post-infection. We found noticeable changes to the isomiR expression profile in response to infection and aging. Data analysis revealed a distinct bias towards isomiR production in the mature miRNA as opposed to the star strand. Furthermore, we noticed that only in 40% of Ae. aegypti miRNAs, the most abundant reads for each particular miRNA match the exact sequence reported in the miRbase. The isomiR expression variations between an Ae. aegypti embryonic cell line (Aag2) and whole mosquitoes demonstrated a tissue-specific pattern of isomiR production. Our results illustrated a bias for certain types of isomiRs for each miRNA. The findings presented in this study also provide evidence that isomiR production is not a random phenomenon and may be important in DENV colonisation of its vector. Examination of isomiR production rate in DENV infected and non infected mosquitoes

2015-11-05 | E-GEOD-59516 | biostudies-arrayexpress

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