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Assemblies of Triticum aestivum (bread wheat) genomic and transcriptomic data.

ABSTRACT: Assemblies of Triticum aestivum (bread wheat) genomic and transcriptomic data.

PROVIDER: PRJEB568 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
		Other
	ERR016397.fastq.gz	Fastqsanger.gz
	ERR016398.fastq.gz	Fastqsanger.gz
	ERR016399.fastq.gz	Fastqsanger.gz

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Project description:Bread wheat (Triticum aestivum) has a large, complex and hexaploid genome consisting of A, B and D homoeologous chromosome sets. Therefore each wheat gene potentially exists as a trio of A, B and D homoeoalleles, each of which may contribute differentially to wheat phenotypes. We describe a novel approach combining wheat cytogenetic resources (chromosome substitution ânullisomic-tetrasomicâ lines) with next generation deep sequencing of gene transcripts (RNA-seq), to directly and accurately identify homoeologue-specific single nucleotide variants and quantify the relative homoeoallelic contribution to gene expression. We obtained mRNA-Seq datasets from non-normalized cDNA libraries created from shoot and root tissues of the euploid bread wheat cultivar Chinese Spring, from which the nullitetra lines are derived, from complete sets of chromosome 1 and 5 nullitetras, and from extant relatives of the diploid A (Triticum urartu) and D (Aegilops tauschii) genome donors, herein referred to as A and D genome diploids

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