GWAS
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB57369 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Despite the fact that taro, colocasia esculenta, is an important staple food for millions of people around the world, its genome and transcriptome sequence has not yet been investigated. The objective of this study was to generate transcriptome sequence information from taro cultivars Niue, Palau 10, and Sam-07. Niue and Sam-07 are highly susceptible to the taro leaf blight (TLB) disease caused by Phytophthora colocasiae, to which Palau 10 is resistant. The analysis of the taro transcriptome will facilitate gene discovery, including genes that are responsible for TLB-resistance. Moreover, microsatellites (SSRs) developped from these data will be useful for marker-assisted breeding of improved taro cultivars, QTL mapping, and characterization of the genetic diversity in taro.
2018-09-30 | GSE87777 | GEO
Project description:Whole genome and transcriptome sequencing of taro, Colocasia esculenta, using next generation sequencing technology
| PRJNA328799 | ENA
Project description:Calcium oxalate raphide crystals are found in bundles in intravacuolar membrane chambers of specialized idioblasts cells of most plant families including many crop plants and are found in most tissues. Raphides are needle-shaped, often with barbed ends, and their morphology and biomineralization pattern have been a focus of much research. In the family Araceae, raphides have been proposed to cause acridity in crops such as taro (Colocasia esculenta (L.) Schott). Acridity is irritation that causes swelling of lips, mouth and throat, as well as itchiness and pain when raw or insufficiently cooked tissues are eaten; this response varies among taro consumers. Since raphides in some other foods do not cause acridity, and since acridity can be inactivated by cooking and/or protease treatment, it is possible that a toxin or allergen-like compound is associated with the crystals. Using two-dimensional (2D) gel electrophoresis and mass spectrometry (MS) peptide sequencing of selected peptides from purified raphides and the results from taro apex transcriptome sequencing, we have shown that profilins, known allergens, are present on raphides. One of the five raphide profilins genes was highly expressed in the apex and had a 17-amino-acid insert that significantly increased that profilin’s epitope peak. This insert was predicted to be coiled and exposed on the surface of the folded peptide. A second profilin had a 2-amino-acid insert that also had a greater B-cell epitope prediction. The taro profilins showed 83 to 92% similarity to known characterized profilins while other raphide peptides showed greater than 90% similarity to other higher plants. The presence on the raphides of peptides normally associated with mitochrondria (ATP synthase), chloroplasts (chaperonin cpn 60 kDa), cytoplasm (actin, profilin) and vacuole (V-type ATPase) indicates a multistage biocrystallation process ending with possible invagination of the tonoplast and addition of mucilage that may be derived from the Golgi. Actin might play a crucial role in the generation of the needle-like raphides. We also showed that commercial allergen test strips for hazelnuts, where profilin is a secondary allergen, have potential for screening in a plant breeding program to reduce acridity and during food processing to avoid over-cooking.
2022-02-19 | GSE191235 | GEO
Project description:Colocasia esculenta Transcriptome or Gene expression
| PRJNA639211 | ENA
Project description:Colocasia esculenta Transcriptome or Gene expression
| PRJNA206412 | ENA
Project description:Colocasia esculenta var. antiquorum Genome sequencing
| PRJNA901400 | ENA
Project description:Next generation sequencing of leaf transcriptome from taro (Colocasia esculenta) cultivars Niue, Palau 10, and Sam-07
| PRJNA347552 | ENA
Project description:Elucidating the origin of Taimo, a cultivar of Taro (Colocasia esculenta) grown in the Ryukyu archipelago, through chloroplast genome analysis
| PRJDB15812 | ENA
Project description:Colocasia esculenta isolate:LXY-2018 Genome sequencing and assembly
| PRJNA587719 | ENA