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Gene expression profiles of migratory and non-migratory B-cells exposed to breast cancer cell derived conditioned medium with and with extracellular vesicles

ABSTRACT: Gene expression profiles of migratory and non-migratory B-cells exposed to breast cancer cell derived conditioned medium with and with extracellular vesicles

PROVIDER: PRJEB57566 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR10484000.fastq.gz	Fastqsanger.gz
	ERR10484001.fastq.gz	Fastqsanger.gz
	ERR10484002.fastq.gz	Fastqsanger.gz
	ERR10484003.fastq.gz	Fastqsanger.gz
	ERR10484004.fastq.gz	Fastqsanger.gz

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Gene expression profiles of migratory and non-migratory B-cells exposed to breast cancer cell derived conditioned medium with and with extracellular vesicles

Project description:Gene expression profiles were generated using RNA-sequencing from migratory and non-migratory B-cells after exposure to conditioned medium of breast cancer cells, either containing or depleted from extracellular vesicles. The purpose of the experiment was to investigate how breast cancer cell derived extracellular vesicles induce specific molecular pathways involved in B-cell migration and B-cell infiltration.

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LC-MSMS of microplasts derived from macrophage conditioned medium treated breast adenocarcinoma MCF-7 cells. Pooja Kamal Melwani, M.Sc., Nishad S, PhD, Badri Narain Pandey, PhD, Radiation Signalling and Cancer Biology Section, Radiation Biology & Health Sciences Division, Bhabha Atomic Research Centre, Mumbai 400 085, India

Project description:Paracrine signals relayed between heterogenous cell types through small and large extracellular vesicles promote cancer cell growth, invasion and metastasis. Microplasts, also called as cytoplasts or cytokineplasts are large extracellular vesicles (0.5-11µm diameter) originating from migratory cells. Microplasts are known to be associated with invasive phenotypes of cancer cells and promote metastasis. Treatment with macrophage conditioned medium induces significant shedding of microplasts from MCF-7 breast adenocarcinoma cells. To characterise microplasts and to study their potential role in intercellular communication in cancer, we isolated microplasts derived from macrophage conditioned medium treated MCF-7 cells and investigated their protein cargo through LC-MSMS.

2021-05-05 | PXD024293 | Pride

Molecular changes induced by melanoma cell conditioned medium (MCM) in HUVEC cells.

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Protein Amount in Conditioned Medium of MenSCs

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Project description:We performed RNA-seq analysis on neural progenitor cells (NPCs) acutely treated (12 hours) with Extracellular vesicles (EVs) purified by the conditioned medium of dorsally patterned and ventrally patterned human cerebral organoids originated from control- or E370K mutant- iPSCs cells. The aim of this experiments was to dissect if EV content leads to transcriptional changes on receiving cells.

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Project description:Conditioned medium (CM) and extracellular vesicles (EV) from human Adipose-derived Stem/stromal cells (ASC) and Dermal fibroblasts (DF) can represent valid cell substitutes in regenerative medicine and other therapeutic applications. Whether one of the two cell products should be preferred over the other is still under debate and no direct comparison of their proteome has been reported yet. Here, we apply a comprehensive quantitative proteomics approach to explore the protein composition of conditioned medium and EV obtained from the two cell types. We identified 1977 proteins by LC-MS/MS proteomic analysis. Unsupervised clustering analysis and PCA clearly distinguished CM and EV as separate groups, regardless the cell source. We identified 68 and 201 proteins that were more abundant or exclusively detectable in CM and EV, respectively. CM were enriched in proteins of endoplasmic reticulum, Golgi apparatus and lysosomes, whereas EV contained a large amount of GTPases, ribosome and translation-related factors. Moreover, the analysis of ASC and DF secretomes (both CM and EV) revealed the presence of cell type-specific factors. ASC-CM and -EV carried factors involved in ECM organization (hyaluronan and glycosaminoglycan metabolism) and immunological regulation (e.g. macrophage and IkB/NFkB signaling regulation), respectively. On the other hand, DF-CM and –EV were both enriched in epithelium development associated factors, whilst DF-CM in proteins involved in cellular processes regulation and -EV in Wnt signaling factors. In conclusion, this comprehensive proteomic analysis of the secretome components of ASC and DF, provides evidence of a different protein composition between CM and EV and of the presence of cell type-specific bioactive mediators suggesting their specific future use as advanced therapy medicinal products.

2020-12-23 | PXD020219 | Pride

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