Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA sequencing of blood and lung neutrophils from tumor-bearing mice

ABSTRACT: RNA sequencing of blood and lung neutrophils from tumor-bearing mice

PROVIDER: PRJEB57588 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other
	ERR10491458_1.fastq.gz	Fastqsanger.gz
	ERR10491458_2.fastq.gz	Fastqsanger.gz
	ERR10491459_1.fastq.gz	Fastqsanger.gz
	ERR10491459_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 11

Similar Datasets

RNA sequencing of blood and lung neutrophils from tumor-bearing mice

Project description:To investigate the difference among neutrophils circulating in the blood, and those infiltrating in the metastatic organ, we isolated peripheral blood (PB) and lung neutrophils from AT3 tumor-bearing mice. Total RNA was isolated from neutrophils and the transcriptional profiles of neutrophils were analyzed by RNA seq.

2022-12-12 | E-MTAB-12365 | biostudies-arrayexpress

Chronic pulmonary bacterial infections facilitate breast cancer lung metastasis with tumor-promoting MHCIIhi neutrophils

Project description:To investigate whether MHCIIhi neutrophils have tumor-promoting properties, we performed RNA-sequencing (RNA-seq) on MHCIIhi neutrophils and MHCIIlow neutrophils isolated from the lung and peripheral blood of tumor-bearing mice.

2023-07-04 | GSE218630 | GEO

Single-cell transcriptional profiles of BM, PB and lung neutrophils from naïve (BALBc/J) and 4T1 tumor-bearing mice

Project description:To better understand how lung neutrophils transcriptionally differ from other tissue neutrophils, we performed single-cell RNA sequencing (scRNA-seq) on BM, PB and lung neutrophils isolated from naïve (BALBc/J) and 4T1 tumor-bearing mice.

2022-12-12 | GSE217143 | GEO

Single-cell RNA-sequencing of tumor associated neutrophils and control peripheral blood neutrophils in a novel lung squamous cell carcinoma mouse model

Project description:Tumor-associated neutrophils (TANs) can be conditioned to become “N2” pro-tumorigenic neutrophils in the tumor microenvironment. TANs have been shown to acquire N2 features and promote multiple aspects of tumor growth in mouse models of many cancers, including non-small cell lung cancer. We developed a novel mouse model for lung squamous cell carcinoma (LSCC): Rosa26LSL-Sox2-IRES-GFP;Nkx2-1fl/fl;Lkb1fl/fl (SNL). SNL mice develop tumors with short latency of ~3 months and SNL tumors have high neutrophil infiltration similar to other LSCC mouse models. We employed this novel model and single-cell RNA-sequencing to profile TANs in SNL lung tumors in comparison to peripheral blood neutrophils (PBNs) from tumor-bearing SNL mice.

2018-10-16 | GSE118245 | GEO

Single-cell transcriptional profiles of BM, PB and lung neutrophils from naïve (C57BL/6J), AT3, and AT3-gcsf tumor-bearing mice

Project description:To determine the effect of tumor-bearing vs. host neutrophilic inflammation on reinforcement of lung neutrophil-specific immunosuppressive function, we performed single-cell RNA sequencing (scRNA-seq) on BM, PB and lung neutrophils isolated from naïve (C57BL/6J), AT3, and AT3-gcsf tumor-bearing mice.

2022-12-12 | GSE217595 | GEO

RNA sequencing of neutrophils residing in bone marrow, peripheral blood and lung

Project description:Generation of organ-infiltrating neutrophils occurs in hematopoietic tissues and organs, such as bone marrow and spleen, in response to tumor- and host-derived factors. The de novo expanded neutrophils then egress from hematopoietic sites, circulate through the blood vessels and infiltrate into the organ interstitia and parenchyma. During above trafficking process, neutrophils can undergo phenotypic and functional changes in response to tissue environments. To determine the difference among neutrophils residing in the hematopoietic site—BM, circulating in the blood, and those infiltrating in the metastatic organ, the transcriptional profiles of neutrophils were analyzed by RNA sequencing. 4T1 cells were injected into the fourth mammary fat pads of female syngeneic BALB/cJ mice (8-week-old, n = 3). At day 10 (pre-metastatic stage), the mice were euthanized and then CD45+CD11b+Ly6G<high>Ly6C<med> neutrophils from bone marrow (BM), peripheral blood (PB) and lung were isolated by fluorescence-activated cell sorting. Total RNA was isolated from neutrophils using the miRNeasy Mini kit (Qiagen) and the transcriptional profiles of neutrophils were analyzed by RNA sequencing

2020-07-24 | E-MTAB-9128 | biostudies-arrayexpress

Chronic pulmonary bacterial infections facilitate breast cancer lung metastasis with tumor-promoting MHCIIhi neutrophils II

Project description:In order to explore whether the tumor-promoting phenotype of MHCIIhi neutrophils was directly affected by bacterial chronic infection, we isolated MHCIIhi neutrophils cells from the lungs of both of CI tumor-bearing mice and CTRL tumor-bearing mice.

2023-07-04 | GSE218646 | GEO

scRNA-seq of lung CD140a+ mesenchymal cells from naïve or tumor-bearing CD140aEGFP reporter mice

Project description:To investigate the CD140a+ mesenchymal cell heterogeneity in the lung in both steady and tumor-bearing conditions, we utilized CD140aEGFP reporter mice, and purified CD140a+ lung mesenchymal cells from naive and tumor-bearing mice and performed scRNA-seq.

2022-11-01 | E-MTAB-11902 | biostudies-arrayexpress

Single-cell transcriptional profiles of different tissue-derived myeloid cells from naïve and MDA-MB-231 tumor-bearing humanized mice

Project description:To better understand how lung neutrophils transcriptionally differ from other tissue neutrophils, we performed single-cell RNA sequencing (scRNA-seq) on different tissue-derived myeloid cells isolated from naïve and MDA-MB-231 tumor-bearing humanized mice.

2022-12-12 | GSE217499 | GEO

scRNA-seq of lung CD140a+ mesenchymal cells from AT3-gcsf tumor-bearing mice

Project description:To characterize lung CD140a+ mesenchymal cells in host neutrophilic inflammation conditions, we isolated lung CD140a+ mesenchymal cells from AT3-gcsf tumor-bearing CD140a-EGFP mice for scRNA-seq.

2023-02-01 | E-MTAB-12371 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data