Project description:Modest target sequencing yield improvements using Nanopore adaptive sampling to reduce human DNA contamination from clinical tissue samples

| PRJEB78709 | ENA

SARS-CoV-2

Project description:Nanopore adaptive sampling with carrier DNA (NASCarD)

| PRJNA930601 | ENA

Nanopore adaptive sampling: a tool for enrichment of low abundance species in metagenomic samples

Project description:Oxford Nanopore sequence data from adaptive sampling experiments using a synthetic bacterial mock community.

| PRJEB44844 | ENA

Adaptive sampling and chromosome sorting Y chromosome data

Project description:Y chromosome chromosome sorting and adaptive sampling data

| PRJEB58141 | ENA

NASCarD (Nanopore Adaptive Sampling with Carrier DNA): A Rapid, PCR-Free Method for SARS-CoV-2 Whole-Genome Sequencing in Clinical Samples.

Project description:Whole-genome sequencing (WGS) represents the main technology for SARS-CoV-2 lineage characterization in diagnostic laboratories worldwide. The rapid, near-full-length sequencing of the viral genome is commonly enabled by high-throughput sequencing of PCR amplicons derived from cDNA molecules. Here, we present a new approach called NASCarD (Nanopore Adaptive Sampling with Carrier DNA), which allows a low amount of nucleic acids to be sequenced while selectively enriching for sequences of interest, hence limiting the production of non-target sequences. Using COVID-19 positive samples available during the omicron wave, we demonstrate how the method may lead to >99% genome completeness of the SARS-CoV-2 genome sequences within 7 h of sequencing at a competitive cost. The new approach may have applications beyond SARS-CoV-2 sequencing for other DNA or RNA pathogens in clinical samples.

| S-EPMC10818518 | biostudies-literature

CDCA7-associated global aberrant DNA hypomethylation translates to localized, tissue-specific transcriptional responses [ONT adaptive sampling]

Project description:CDCA7-associated global aberrant DNA hypomethylation translates to localized, tissue-specific transcriptional responses [ONT adaptive sampling]

| PRJNA1043620 | ENA

Carrier Subclass

Project description:Carrier Subclass

| PRJNA1031099 | ENA