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Transcriptional profiling of murine distal colon from C57BL/6 (WT) and Stat2KO mice during acute DSS colitis.

ABSTRACT: Transcriptional profiling of murine distal colon from C57BL/6 (WT) and Stat2KO mice during acute DSS colitis.

PROVIDER: PRJEB60040 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR10905147_1.fastq.gz	Fastqsanger.gz
	ERR10905147_2.fastq.gz	Fastqsanger.gz
	ERR10905148_1.fastq.gz	Fastqsanger.gz
	ERR10905148_2.fastq.gz	Fastqsanger.gz
	ERR10905149_1.fastq.gz	Fastqsanger.gz

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Transcriptional profiling of murine distal colon from C57BL/6 (WT) and Stat2KO mice during acute DSS colitis.

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Project description:To find out which miRNAs are significantly differential expression and potentially involved in the process of inflammation promoting carcinogenesis of colorectal cancer (CRC). We established a colitis-associated CRC (AOM/DSS, Azoxymethane/Dextran sulfate sodium salt) model, colitis (DSS) model and high dose carcinogen (AOM, about 5 times AOM amount given than AOM/DSS model) model. At day 100 when tumor formed in AOM/DSS bearing mice (colitis-associated CRC mice) but no tumor was found in AOM (high dose carcinogen) and DSS model, we employed miRNA microarray as a discovery platform to identify genes with the potential to involve in the progression of CRC promoted by inflammation. 5-7 weeks female BALB/c mice, (1) AOM/DSS group: AOM 12.5mg/kg i.p. at day 1, DSS drinking 5d/21dx3circles from day 5; (2) AOM group: AOM 10mg/kg i.p. 1/weekx6 from day 1; (3) DSS group: DSS drinking 5d/21dx3circles from day 5. The distal colon epithelial tissues were collected at day100 when tumor formed in AOM/DSS bearing mice. The miRNA microarray experiments were performed together.

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