Dataset Information

Metagenomic sequences from post-processing waters from underground coal gasification process (UCG)

ABSTRACT: UCGWATERplus aims at remediating waters polluted with organic and inorganic contaminants as a result of the operation of underground coal gasification (UCG) plants. Three remediation strategies are contemplated: (1) (electro)coagulation, (2) the use of polymer- and carbon-based adsorbents derived from coal by-products and residues, thus contributing to revalorization of the latter as a positive side-effect of the action, and (3) bioremediation based on constructed wetlands. Combinations of these methods are explored for maximum decontamination efficiency, while also analyzing their technical and economic feasibility. The utility of the developed materials and decontamination strategies beyond UCG water remediation is addressed as well.

PROVIDER: PRJEB60074 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR10908575_1.fastq.gz	Fastqsanger.gz
	ERR10908575_2.fastq.gz	Fastqsanger.gz
	ERR10908576_1.fastq.gz	Fastqsanger.gz
	ERR10908576_2.fastq.gz	Fastqsanger.gz
	ERR10908577_1.fastq.gz	Fastqsanger.gz

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Project description:We used the mummichog (Fundulus heteroclitus) array we developed to test whether our arrays could be used to monitor the efficacy of remediation at an estuarine Superfund site. Shipyard Creek is a chromium-contaminated Superfund site in Charleston, SC undergoing remediation, therefore it provides a unique opportunity to study the efficacy of arrays as a molecular biomarker in of toxicant effects in mummichogs. Mummichogs were captured in Shipyard Creek in Charleston, SC prior to remediation (2000), after remediation began (2003), and as remediation further progressed (2005). Simultaneously, mummichogs were collected from a reference site at the Winyah-Bay National Estuarine Research Reserve (NERR). The hepatic gene expression pattern of fish captured at Shipyard Creek showed wide differences from the fish captured at NERR in 2000. As remediation progressed the gene expression pattern of fish captured at Shipyard Creek became increasingly similar to fish captured at NERR, and the number of genes differently expressed dropped from 22 to 4. The magnitude of differential gene expression of the individual genes also decreased during remediation. The recovering gene expression profile is associated with lower chromium bioavailability, demonstrated through significantly decreased body burden and sediment concentrations. For example, sediment concentrations at Shipyard Creek were 80-fold greater than NERR in 2000, 51-fold greater in 2003, and only 8-fold greater in 2005. However, hydraulic dredging in 2005 stirred up the sediments and increased body burden of chromium even though chromium sediment concentrations continued to drop. Therefore, the number of differentially expressed genes increased to 9. Overall, the data supports our hypothesis that arrays can be used to monitor site mitigation, as the number of genes differentially expressed mimics the body burden and also indicates when on-site remediation is increasing bioavailability. Keywords: Field site

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Metagenomic sequences from post-processing waters from underground coal gasification process (UCG)

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