Project description:In present study, it was found that the co-culture of Wickerhamomyces anomalus Y-5 and L. plantarum RX-8 could enhance bacteriocin production. To analyze the interaction between W. anomalus Y-5 and L. plantarum RX-8, a quantitative proteomic approach was used to analyze and compare the proteome in L. plantarum RX-8 and W. anomalus Y-5 under mono-culture and co-culture. In total, 339 differently expressed proteins (DEPs) were screened in comparison of L. plantarum RX-8 under mono-culture and co-culture, 645 proteins of W. anomalus Y-5 changed in mono-culture and co-culture. The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis indicated that DEPs participated in various metabolic pathways such as PTS system, glycolysis, galactose metabolism, glutamate, aspartate, arginine and cysteine metabolism etc. These pathways were related to inducing mechanism on improving bacteriocin production by co-culture. Quantitative proteomic analysis-based strategies can therefore provide further evidence for new regulated targets to improve the production of bacteriocins.
Project description:This is genome-scale metabolic model of Cyberlindnera americana as the representative yeast species for the clade Phaffomycetaceae. This model was generated through homology search using a fungal pan-GEM largely based on Yeast8 for Saccharomyces cerevisiae, in addition to manual curation. This model has been produced by the Yeast-Species-GEMs project from Sysbio (www.sysbio.se). This is model version 1.0.0 accompanying the publication (DOI: 10.15252/msb.202110427), currently hosted on BioModels Database and identified by MODEL2109130008. Further curations of this model will be tracked in the GitHub repository: https://github.com/SysBioChalmers/Yeast-Species-GEMs Models for species of the same clade includes: Cyberlindnera fabianii; Cyberlindnera jadinii; Wickerhamomyces anomalus; Wickerhamomyces ciferrii; Starmera quercuum; Candida stellimalicola; Candida freyschussii; Cyberlindnera mrakii; Cyberlindnera misumaiensis; Candida montana; Barnettozyma californica; Barnettozyma hawaiiensis; Barnettozyma populi; Barnettozyma pratensis; Barnettozyma salicaria; Candida ponderosae; Wickerhamomyces hampshirensis; Wickerhamomyces bovis; Wickerhamomyces alni; Wickerhamomyces sp.; Cyberlindnera petersonii; Wickerhamomyces canadensis; Starmera amethionina; Phaffomyces opuntiae; Phaffomyces antillensis; Phaffomyces thermotolerans; Candida orba; Cyberlindnera saturnus; Cyberlindnera suaveolens; Cyberlindnera xylosilytica; Candida mycetangii; Candida vartiovaarae; Cyberlindnera maclurae; Cyberlindnera americana. These models are available in the zip file. To cite BioModels, please use: V Chelliah et al; BioModels: ten-year anniversary. Nucleic Acids Res 2015; 43 (D1): D542-D548. To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to MIT License for more information.
Project description:We compare the transcriptome of gnotobiotic Ae. aegypti generated by contaminating axenic (bacteria-free) larvae with bacterial isolates found in natural mosquito breeding sites. We focused on four bacterial isolates (Lysobacter, Flavobacterium, Paenibacillus and Enterobacteriaceae) and found that different gnotobiotic treatments resulted in massive transcriptomic changes throughout the mosquito development.