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Sponge transcriptomic response to bacterioplankton vs. symbiotic microbes

ABSTRACT: Transcriptomic data of two Mediterranean sponges exposed to bacterioplankton and symbiont consortia

PROVIDER: PRJEB61098 | ENA |

REPOSITORIES: ENA

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Sponge transcriptomic response to bacterioplankton and symbiotic microbes

Project description:Molecular response of Mediterranean sponges upon encounter with bacterioplankton and symbiont bacteria consortia

| PRJEB61959 | ENA

Transcriptomic profiles of two sponge species upon exposure to microbial-associated molecular patterns (lipopolysaccharide and peptidoglycan) compared against control (sterile artificial seawater)

Project description:The interaction of animals with microbes relies on the specific recognition of microbial-derived molecules by receptors of the immune system. Sponges (phylum Porifera), as sister group of the Eumetazoa, provide insights into conserved mechanisms for animal-microbe crosstalk, but empirical data is limited. Here we aimed to characterize the immune response of sponges upon microbial stimuli by RNA-Seq. Two sponges species from the Mediterranean Sea, Aplysina aerophoba and Dysidea avara, were challenged with microbial-associated molecular patterns (lipopolysaccharide and peptidoglycan) or sterile artificial seawater (control) in aquarium experiments. Sponge tissue samples were collected 1h, 3h, and 5h after treatment. The response of the sponges to the treatments was assessed by differential gene expression analysis of RNA-Seq data. For each species, we compared the transcriptomic profiles of the samples in MAMP treatment to control within each time point.

2018-11-26 | E-MTAB-6757 | biostudies-arrayexpress

Sponge symbiotic bacterial communities metagenome

Project description:Screening of disease-associated microbiomes of Lubomirskia baicalensis sponges

| PRJNA480187 | ENA

Microbes associated with Dictyoceratida sponges from Crete, Greece

Project description:Microbial diversity in four Mediterranean irciniid sponges

| PRJEB23640 | ENA

Characterization of a thaumarchaeal symbiont that drives incomplete nitrification in the tropical sponge Ianthella basta

Project description:Marine sponges represent one of the few eukaryotic groups that ubiquitously harbor symbiotic members of the Thaumarchaeota, which are important chemoautotrophic ammonia-oxidizers in many environments. However in most studies, direct demonstration of ammonia-oxidation by these archaea within sponges is lacking, and little is known about sponge-specific adaptations of archaeal ammonia oxidizers (AOA). In this study, we characterized the thaumarchaeal symbiont of the marine sponge Ianthella basta using metaproteogenomics, fluorescence in situ hybridization, qPCR and direct isotope-based functional assays. We demonstrate that the I. basta symbiont is not closely related to other genomically sequenced sponge AOA and is a member of a new genus. “Candidatus Nitrosospongia bastadiensis” is an abundant symbiont that is solely responsible for nitrite formation from ammonia in I. basta that surprisingly does not harbor nitrite-oxidizing microbes. Consistently, Ca N. bastadiensis encodes and expresses the genetic repertoire required for chemolithoautotrophic ammonia oxidation. Furthermore, we show that this AOA is equipped with an expanded set of extracellular subtilisin-like proteases, a metalloprotease unique among archaea, as well as a putative branched-chain amino acid ABC transporter. This repertoire is strongly indicative of a mixotrophic lifestyle and is (with slight variations) also found in other sponge-associated, but not in free-living AOA. We predict that this feature as well as an expanded and unique set of secreted serpins (protease inhibitors), a unique array of eukaryotic-like proteins, and a DNA-phosporothioation system likely involved in defense against foreign DNA, represent important adaptations of AOA to life within these ancient filter-feeding animals.

2019-11-13 | PXD012484 | Pride

Sponge-associated microbes from Lembeh

Project description:This study aimed to investigate the diversity of microorganism in sponges from Lembeh

| PRJEB27889 | ENA

bacterioplankton metagenome

Project description:bacterioplankton metagenome Metagenome

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bacterioplankton community

Project description:bacterioplankton community Metagenome

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sponge metagenome

Project description:Sponges Metagenome

| PRJNA325781 | ENA

sponge development

Project description:In order to compare sponge and eumetazoan (higher animal) body plans, we identified and studied expression of a broad range of eumetazoan developmental regulatory genes in Sycon ciliatum (Calcispongiae). In this species, embryonic development is semi-synchronous within a population, synchronous within individuals, and oocytes and embryos occupy a significant fraction of the volume of the sponges during the reproductive period. RNASeq libraries representing non-reproductive (somatic) tissue slices along the body axis, as well as oocytes, embryos and free swimming larvae were generated from material obtained by sampling throughout the life cycle.

2014-05-01 | E-MTAB-2431 | biostudies-arrayexpress

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