Project description:<p>Marine dissolved organic matter (DOM) varies in its recalcitrance to rapid microbial degradation. DOM of varying recalcitrance can be exported from the ocean surface to depth by subduction or convective mixing and oxidized over months to decades in deeper seawater. Carboxyl-rich alicyclic molecules (CRAM) are characterized as a major component of recalcitrant DOM throughout the oceanic water column. The oxidation of CRAM-like compounds may depend on specific bacterioplankton lineages with oxidative enzymes capable of catabolizing complex molecular structures like long-chain aliphatics, cyclic alkanes, and carboxylic acids. To investigate the interaction between bacteria and CRAM-like compounds, we conducted microbial remineralization experiments using several compounds rich in carboxyl groups and/or alicyclic rings, including deoxycholate, humic acid, lignin, and benzoic acid, as proxies for CRAM. Mesopelagic seawater (200 m) from the northwest Sargasso Sea was used as media and inoculum and incubated over 28 days. All amendments demonstrated significant DOC removal (2 – 11 µmol C L-1) compared to controls. Bacterioplankton abundance increased significantly in the deoxycholate and benzoic acid treatments relative to controls, with fast-growing <em>Spongiibacteracea</em>, <em>Euryarcheaota</em>, and slow-growing SAR11 enriched in the deoxycholate treatment and fast-growing <em>Alteromonas</em>, <em>Euryarcheaota</em>, and <em>Thaumarcheaota</em> enriched in the benzoic acid treatment. In contrast, bacterioplankton grew slower in the lignin and humic acid treatments, with oligotrophic SAR202 becoming significantly enriched in the lignin treatment. Our results indicate that the character of the CRAM proxy compounds resulted in distinct bacterioplankton removal rates and affected specific lineages of bacterioplankton capable of responding.</p>
Project description:The goal of the study was to identify miRNAs differentially expressed in the invasive front of early and more advanced colorectal cancers, and to determine wheter miRNA profiles could differentiate between colorectal cancers with and without early metasttic progression. miRNA array data was used for analyses of associations beteen metastatic status and for associations to histopathological risk factors for metastatic progression.
Project description:Invasive tumor front (the tumor-host interface) is vitally important in malignant cell progression and metastasis. Tumor cell interactions with resident and infiltrating host cells and with the surrounding extracellular matrix and secreted factors ultimately determine the fate of the tumor. In this study we focus on the invasive tumor front, making an in-depth characterization of reticular fiber scaffolding, infiltrating immune cells, gene expression and methylation profiles of classified aggressive primary uterine adenocarcinomas and leiomyosarcomas.
Project description:Invasive tumor front (ITF, the tumor-host interface) is vitally important in malignant cell progression and metastasis. Tumor cell interactions with resident and infiltrating host cells and with the surrounding extracellular matrix and secreted factors ultimately determine the fate of the tumor. Herein we focus on the invasive tumor front, making an in-depth characterization of reticular fiber scaffolding, infiltrating immune cells, gene expression and epigenetic profiles of classified aggressive primary uterine adenocarcinomas (uADC) and leiomyosarcomas (uLMS).
Project description:We have characterized the transcriptional program regulated by ETV5 at the invasive front of endometrial carcinomas, by comparing it with non-invasive areas of the same tumor samples.
2014-11-05 | GSE57870 | GEO
Project description:16S rDNA gene sequences belonging to bacterioplankton
Project description:The goal was to identify gene expression signatures predictive of early relapse among stage IIA/MSS colon cancer patients. A secondary question addressed was whether the invasion front profiles could generate a competitive signature. 39 whole tumor and 35 matched invasion front profiles were generated (4 samples failed) and grouped into \\"early relapse\\" (relapse within 5 years) and \\"no relapse\\" (no relapse for at least 6 years) categories.
