Project description:The species-specific identification of fibre origin is essential in archaeology but reveals challenging for closely related species. This is particularly true between the four South American Camelids (SAC) species: alpaca, guanaco, llama and vicuña. The analysis of proteins extracted from hairs and/or yarns by proteomics has emerged as a powerful method to differentiate between species. However, for SAC, the database information available is very poor, which limits this approach. In this study, we analysed 42 modern and 4 archaeological reference samples from the four SAC species.
Project description:We performed a genome wide methylation scan using the Illumina HumanMethylation27 BeadChip on DNAs extracted from the leukocytes of 8 hypertensive cases and 8 normotensive age-matched controls. All subjects were African American males aged 14-23 years.
Project description:We performed a genome wide methylation scan using the Illumina HumanMethylation27 BeadChip on DNAs extracted from the leukocytes of 7 obese (BMI≥99th percentile for age and sex) and 7 lean (BMI≤10th percentile for age and sex) African American males aged 14-18 years.
Project description:A population and admixture analysis of Mesoamerican Totonacs and South American Bolivians. A panel of highly informative ancestry informative markers (AIMs) for New World populations is identified. Regions coinciding with AIMs are have moderate signatures of selection. Population structure and differentiation were assessed with a genome-wide panel of 815,377 autosomal markers, Y-chromosome STR and SNPs, and mtDNA sequence data.
Project description:To define the genome-wide activity of Cas9-sgRNA directed at the gamma-globin promoter genes, we performed CHANGE-seq and identified 678 off-target sites from plerixafor-mobilized normal donors of African American ancestry (pre-GMP engineering runs, n = 3). Most of these off-targets are in intergenic and intronic regions of the human genome, as expected based on their relative genomic proportions. We selected the 277 CHANGE-seq candidate sites reproducibly identified in 2 or more biological donors and in silico predicted sites for multiplexed targeted amplicon sequencing (rhAmp-seq, IDT).
Project description:We performed a genome wide methylation scan using the Illumina HumanMethylation27 BeadChip on DNAs extracted from the leukocytes of 8 hypertensive cases and 8 normotensive age-matched controls. All subjects were African American males aged 14-23 years. Bisulphite converted DNA from the 16 samples were hybridised to the Illumina Infinium 27k Human Methylation Beadchip v1.2