Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

De-novo genome assembly of the invasive mosquito species Aedes japonicus and Aedes koreicus

ABSTRACT: De-novo genome assembly of the invasive mosquito species Aedes japonicus and Aedes koreicus

PROVIDER: PRJEB61905 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Aedes koreicus and Aedes japonicus

Project description:De-novo genome of the invasive mosquito species Aedes japonicus and Aedes koreicus

| PRJNA948175 | ENA

Aedes koreicus

Project description:De-novo genome of the invasive mosquito species Aedes japonicus and Aedes koreicus

| PRJNA947548 | ENA

Aedes japonicus

Project description:De-novo genome of the invasive mosquito species Aedes japonicus and Aedes koreicus

| PRJNA947978 | ENA

Aedes koreicus

Project description:Aedes koreicus overview

| PRJNA624699 | ENA

De novo genome assembly of the invasive mosquito species Aedes japonicus and Aedes koreicus.

Project description:BackgroundRecently, two invasive Aedes mosquito species, Ae. japonicus and Ae. koreicus, are circulating in several European countries posing potential health risks to humans and animals. Vector control is the main option to prevent mosquito-borne diseases, and an accurate genome sequence of these mosquitoes is essential to better understand their biology and to develop effective control strategies.MethodsA de novo genome assembly of Ae. japonicus (Ajap1) and Ae. koreicus (Akor1) has been produced based on a hybrid approach that combines Oxford Nanopore long-read and Illumina short-read data. Their quality was ascertained using various metrics. Masking of repetitive elements, gene prediction and functional annotation was performed.ResultsSequence analysis revealed a very high presence of repetitive DNA and, among others, thermal adaptation genes and insecticide-resistance genes. Through the RNA-seq analysis of larvae and adults of Ae. koreicus and Ae. japonicus exposed to different temperatures, we also identified genes showing a differential temperature-dependent activation.ConclusionsThe assembly of Akor1 and Ajap1 genomes constitutes the first updated collective knowledge of the genomes of both mosquito species, providing the possibility of understanding key mechanisms of their biology such as the ability to adapt to harsh climates and to develop insecticide-resistance mechanisms.

| S-EPMC10658958 | biostudies-literature

Aedes koreicus

Project description:Microbiota of Aedes koreicus across developmental stages

| PRJNA549354 | ENA

Aedes koreicus mitogenomes

Project description:First finding of Aedes koreicus (Diptera: Culicidae) in the Netherlands, Europe

| PRJEB49267 | ENA

Multifaceted Functional Implications of an Endogenously Expressed tRNA Fragment in Vector Mosquito Aedes aegypti

Project description:We performed small RNA sequencing (Illumina MiSeq) of mosquito Aedes aegypti to profile expression of tRNA derived fragments.

2018-01-03 | GSE101956 | GEO

MLST of Wolbachia isolated from Aedes koreicus

Project description:MLST of Wolbachia isolated from Aedes koreicus

| PRJEB48737 | ENA

Development of microsatellite markers for the invasive mosquito Aedes koreicus.

Project description:BackgroundAedes koreicus is a mosquito species native to East Asia which has recently invaded several countries in Europe. In Italy, this mosquito was first detected in the North-East in 2011 and is now widely distributed in the entire northern part of the country. The development of specific genetic markers, such as microsatellites, is necessary to uncover the dispersal routes of this mosquito from its native areas and, eventually, to plan future control interventions.MethodsAvailable raw sequences of genomic DNA of Ae. koreicus were screened in silico using BLASTn to identify possible microsatellite-containing sequences. Specific primer pairs were then designed, and their efficiency was determined through polymerase chain reaction (PCR) on 32 individuals of Ae. koreicus collected in Italy. PCR conditions were optimised in three multiplex reactions. Genotyping of individual mosquitoes was performed on both single and multiplex PCR reactions. Finally, analysis of intra-population variation was performed to assess the level of polymorphism of the markers.ResultsMosquito genotyping provided consistent results in both single and multiplex reactions. Out of the 31 microsatellite markers identified in the Ae. koreicus genome raw sequences, 11 were polymorphic in the examined mosquito samples.ConclusionsThe results show that the 11 microsatellite markers developed here hold potential for investigating the genetic structure of Ae. koreicus populations. These markers could thus represent a novel and useful tool to infer the routes of invasion of this mosquito species into Europe and other non-native areas.

| S-EPMC10324130 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data